TY - JOUR
T1 - Miktoarm star polymers nanocarrier
T2 - synthesis, characterisation, and in-vitro drug release study
AU - Chong, Yie Kie
AU - Zainol, Ismail
AU - Ng, Chew Hee
AU - Ooi, Ing Hong
N1 - Funding Information:
Acknowledgements The authors would like to thank the Ministry of Higher Education Malaysia (MOHE) for financial support through Fundamental Research Grant Scheme FRGS/1/2013/ST05/ IMU /02/1 and to IMU for a graduate assistantship to Yie Kie Chong.
Publisher Copyright:
© 2019, The Polymer Society, Taipei.
PY - 2019/2/28
Y1 - 2019/2/28
N2 - Conjugation of poly(ethylene glycol) (PEG) to poly(lactide-co-glycolide) (PLGA) renders the latter with enhanced biocompatibility and broader overall capability in biomedical application. Novel miktoarm star polymers comprising PLGA and PEG segments are of interest for their potential as drug carriers. Thus, a series of miktoarm star copolymers, PLGA-(mPEG) 2 , with different PLGA arm molecular weights and methoxy-PEG (mPEG) arm (2000 g/mol), were synthesised via a four-step reaction using carbodiimide chemistry with a low steric hindrance trifunctional linker aminoadipic acid (AAA) and characterised by proton nuclear magnetic resonance ( 1 H NMR), fourier transform infrared (FTIR), gel permeation chromatography (GPC) and differential scanning calorimetry (DSC). Results show that the miktoarm star polymers PLGA 17.0 -AAA(mPEG) 2 and PLGA 43.4 -AAA(mPEG) 2 formed stable nanoparticles and PLGA 4.6 -AAA(mPEG) 2 self-assembled into stable nanomicelles. Fluorescence spectroscopy showed that the critical micelle concentration of PLGA 4.6 -AAA(mPEG) 2 was very low at 6.03 × 10 −7 g/mL. Model drug ibuprofen encapsulated nanoparticles and nanomicelles had good drug loading, high encapsulation efficiency, narrow size distribution, and spherical morphology with negative surface charges. The mean particle size increased with increasing PLGA molecular weights, from 37.28 ± 1.03 to 151.5 ± 0.86 nm. In-vitro release of model drug ibuprofen over 7 days from PLGA 43.4 -AAA(mPEG) 2 nanoparticles (61.65 ± 3.04%) was higher than those of PLGA 4.6 -AAA(mPEG) 2 nanomicelles (26.93 ± 1.49%) and PLGA 17.0 -AAA(mPEG) 2 nanoparticles (10.57 ± 0.29%), with all demonstrating controlled release characteristics. In conclusion, the novel miktoarm star polymers PLGA 43.4 -AAA(mPEG) 2 and PLGA 17.0 -AAA(mPEG) 2 and their nanoparticles, and PLGA 4.6 -AAA(mPEG) 2 and its nanomicelles have a great potential as a nanocarrier for controlled delivery of hydrophobic drugs.
AB - Conjugation of poly(ethylene glycol) (PEG) to poly(lactide-co-glycolide) (PLGA) renders the latter with enhanced biocompatibility and broader overall capability in biomedical application. Novel miktoarm star polymers comprising PLGA and PEG segments are of interest for their potential as drug carriers. Thus, a series of miktoarm star copolymers, PLGA-(mPEG) 2 , with different PLGA arm molecular weights and methoxy-PEG (mPEG) arm (2000 g/mol), were synthesised via a four-step reaction using carbodiimide chemistry with a low steric hindrance trifunctional linker aminoadipic acid (AAA) and characterised by proton nuclear magnetic resonance ( 1 H NMR), fourier transform infrared (FTIR), gel permeation chromatography (GPC) and differential scanning calorimetry (DSC). Results show that the miktoarm star polymers PLGA 17.0 -AAA(mPEG) 2 and PLGA 43.4 -AAA(mPEG) 2 formed stable nanoparticles and PLGA 4.6 -AAA(mPEG) 2 self-assembled into stable nanomicelles. Fluorescence spectroscopy showed that the critical micelle concentration of PLGA 4.6 -AAA(mPEG) 2 was very low at 6.03 × 10 −7 g/mL. Model drug ibuprofen encapsulated nanoparticles and nanomicelles had good drug loading, high encapsulation efficiency, narrow size distribution, and spherical morphology with negative surface charges. The mean particle size increased with increasing PLGA molecular weights, from 37.28 ± 1.03 to 151.5 ± 0.86 nm. In-vitro release of model drug ibuprofen over 7 days from PLGA 43.4 -AAA(mPEG) 2 nanoparticles (61.65 ± 3.04%) was higher than those of PLGA 4.6 -AAA(mPEG) 2 nanomicelles (26.93 ± 1.49%) and PLGA 17.0 -AAA(mPEG) 2 nanoparticles (10.57 ± 0.29%), with all demonstrating controlled release characteristics. In conclusion, the novel miktoarm star polymers PLGA 43.4 -AAA(mPEG) 2 and PLGA 17.0 -AAA(mPEG) 2 and their nanoparticles, and PLGA 4.6 -AAA(mPEG) 2 and its nanomicelles have a great potential as a nanocarrier for controlled delivery of hydrophobic drugs.
KW - Ibuprofen
KW - In vitro release
KW - Miktoarm star polymers
KW - Nanoparticles
KW - PEG
KW - PLGA
UR - http://www.scopus.com/inward/record.url?scp=85062284723&partnerID=8YFLogxK
U2 - 10.1007/s10965-019-1726-4
DO - 10.1007/s10965-019-1726-4
M3 - Article
AN - SCOPUS:85062284723
VL - 26
JO - Journal of Polymer Research
JF - Journal of Polymer Research
SN - 1022-9760
IS - 3
M1 - 79
ER -