Migratory Dendritic Cells Transfer Antigen to a Lymph Node-Resident Dendritic Cell Population for Efficient CTL Priming

Rhys S. Allan, Jason Waithman, Sammy Bedoui, Claerwen M. Jones, Jose A. Villadangos, Yifan Zhan, Andrew M Lew, Ken Shortman, William R. Heath, Francis R. Carbone

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496 Citations (Scopus)

Abstract

Skin dendritic cells (DCs) are thought to act as key initiators of local T cell immunity. Here we show that after skin infection with herpes simplex virus (HSV), cytotoxic T lymphocyte (CTL) activation required MHC class I-restricted presentation by nonmigratory CD8+ DCs rather than skin-derived DCs. Despite a lack of direct presentation by migratory DCs, blocking their egress from infected skin substantially inhibited class I-restricted presentation and HSV-specific CTL responses. These results support the argument for initial transport of antigen by migrating DCs, followed by its transfer to the lymphoid-resident DCs for presentation and CTL priming. Given that relatively robust CTL responses were seen with small numbers of skin-emigrant DCs, we propose that this inter-DC antigen transfer functions to amplify presentation across a larger network of lymphoid-resident DCs for efficient T cell activation.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalImmunity
Volume25
Issue number1
DOIs
Publication statusPublished - 1 Jul 2006
Externally publishedYes

Keywords

  • CELLIMMUNO

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