Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms

Carin Black; Ahmed Al-Amin; Daniel Lorber Rolnik; Stefan C. Kane; Caroline Stolarek; Adrienne White; Fabricio da Silva Costa; Shaun Brennecke

doi:10.1016/j.placenta.2019.08.083

Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms

Carin Black, Ahmed Al-Amin, Daniel Lorber Rolnik, Stefan C. Kane, Caroline Stolarek, Adrienne White, Fabricio da Silva Costa, Shaun Brennecke

Obstetrics & Gynaecology Monash Health

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19–22 weeks’ gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.

Original language	English
Pages (from-to)	11-14
Number of pages	4
Journal	Placenta
Volume	86
DOIs	https://doi.org/10.1016/j.placenta.2019.08.083
Publication status	Published - Oct 2019

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10.1016/j.placenta.2019.08.083

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Black, Carin ; Al-Amin, Ahmed ; Rolnik, Daniel Lorber ; Kane, Stefan C. ; Stolarek, Caroline ; White, Adrienne ; da Silva Costa, Fabricio ; Brennecke, Shaun. / Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF) : An inter-assay comparison of three automated immunoassay platforms. In: Placenta. 2019 ; Vol. 86. pp. 11-14.

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title = "Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms",

abstract = "We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19–22 weeks{\textquoteright} gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.",

author = "Carin Black and Ahmed Al-Amin and Rolnik, {Daniel Lorber} and Kane, {Stefan C.} and Caroline Stolarek and Adrienne White and {da Silva Costa}, Fabricio and Shaun Brennecke",

year = "2019",

month = oct,

doi = "10.1016/j.placenta.2019.08.083",

language = "English",

volume = "86",

pages = "11--14",

journal = "Placenta",

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publisher = "Elsevier - WB Saunders",

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Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF) : An inter-assay comparison of three automated immunoassay platforms. / Black, Carin; Al-Amin, Ahmed; Rolnik, Daniel Lorber; Kane, Stefan C.; Stolarek, Caroline; White, Adrienne; da Silva Costa, Fabricio ; Brennecke, Shaun.

In: Placenta, Vol. 86, 10.2019, p. 11-14.

Research output: Contribution to journal › Article › Research › peer-review

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AB - We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19–22 weeks’ gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.

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