Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms

Carin Black, Ahmed Al-Amin, Daniel Lorber Rolnik, Stefan C. Kane, Caroline Stolarek, Adrienne White, Fabricio da Silva Costa, Shaun Brennecke

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1 Citation (Scopus)

Abstract

We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19–22 weeks’ gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.

Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalPlacenta
Volume86
DOIs
Publication statusPublished - Oct 2019

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