Microvalves for integrated selective droplet generation, splitting and merging on a chip

Sagar N. Agnihotri, Mohammad Reza Raveshi, Rajneesh Bhardwaj, Adrian Neild

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

In this paper, we demonstrate a selective multisite splitting and merging system to perform the type of permutations of reactions required for high-throughput screening. Our approach uses multiple single-layer microfluidic valves as they offer simplicity, biocompatibility, and high-speed operation. These valves are used both to control the generation of droplets and the location of splitting. In the former, the valves are used to completely block some microchannels; in the latter, they create a more modest local geometry variation. The generated droplets act as a vessel that transports different samples around the chip; these vessels can then be subdivided at splitting locations, just as a pipette can be used to dispense a fraction of its total volume. The split droplets enter a merging channel, in which daughter droplets of different types can be merged. We demonstrate the merging of different droplet types in different concentrations at four merging locations.

Original languageEnglish
Article number88
Number of pages13
JournalMicrofluidics and Nanofluidics
Volume25
Issue number11
DOIs
Publication statusPublished - 23 Sept 2021

Keywords

  • Droplet generation
  • Droplet merging
  • Droplet microfluidics
  • Droplet splitting
  • Single-layer microvalves

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