Microstamp patterning of protein arrays

Mahyar Nasabi; Arnan Mitchell; Kourosh Kalantar-Zadeh; Warwick S. Nesbitt

doi:10.1109/ICONN.2008.4639260

Microstamp patterning of protein arrays

Mahyar Nasabi
, Arnan Mitchell
, Kourosh Kalantar-Zadeh
, Warwick S. Nesbitt

Australian Centre for Blood Diseases

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

1 Citation (Scopus)

Abstract

Pathological thrombus formation is initiated by the interaction of blood platelets to immobilized proteins at the vessel wall. Platelet surface adhesion leads to biochemical activation and structural reorganization resulting in spreading of the platelet across the adhesive surface. Extensive studies have been carried out to examine platelet spreading responses on continuous substrates but little is known about the impact of protein surface distribution on platelet function. This paper describes the development of a micro-contact printing technique to establish defined 2-dimensional arrays of the thrombogenic protein fibrinogen at the surface of glass substrates, with the aim of investigating the impact of protein surface distribution on platelet biochemical signaling events associated with the adhesion/spreading process.

Original language	English
Title of host publication	Proceedings of the 2008 International Conference on Nanoscience and Nanotechnology, ICONN 2008
Publisher	IEEE, Institute of Electrical and Electronics Engineers
Pages	117-120
Number of pages	4
ISBN (Print)	1424415047, 9781424415045
DOIs	https://doi.org/10.1109/ICONN.2008.4639260
Publication status	Published - 24 Nov 2008
Event	International Conference on Nanoscience and Nanotechnology (ICONN) 2008 - Melbourne Convention Centre, Melbourne, Australia Duration: 25 Feb 2008 → 29 Feb 2008 Conference number: 2nd

Conference

Conference	International Conference on Nanoscience and Nanotechnology (ICONN) 2008
Abbreviated title	ICONN 2008
Country/Territory	Australia
City	Melbourne
Period	25/02/08 → 29/02/08

Keywords

Fibrinogen
Microstamp
PDMS
Platelet

Access to Document

10.1109/ICONN.2008.4639260

Cite this

@inproceedings{76b79149e30d402889e59bd5e6ce9985,

title = "Microstamp patterning of protein arrays",

abstract = "Pathological thrombus formation is initiated by the interaction of blood platelets to immobilized proteins at the vessel wall. Platelet surface adhesion leads to biochemical activation and structural reorganization resulting in spreading of the platelet across the adhesive surface. Extensive studies have been carried out to examine platelet spreading responses on continuous substrates but little is known about the impact of protein surface distribution on platelet function. This paper describes the development of a micro-contact printing technique to establish defined 2-dimensional arrays of the thrombogenic protein fibrinogen at the surface of glass substrates, with the aim of investigating the impact of protein surface distribution on platelet biochemical signaling events associated with the adhesion/spreading process.",

keywords = "Fibrinogen, Microstamp, PDMS, Platelet",

author = "Mahyar Nasabi and Arnan Mitchell and Kourosh Kalantar-Zadeh and Nesbitt, \{Warwick S.\}",

year = "2008",

month = nov,

day = "24",

doi = "10.1109/ICONN.2008.4639260",

language = "English",

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pages = "117--120",

booktitle = "Proceedings of the 2008 International Conference on Nanoscience and Nanotechnology, ICONN 2008",

publisher = "IEEE, Institute of Electrical and Electronics Engineers",

address = "United States of America",

note = "International Conference on Nanoscience and Nanotechnology (ICONN) 2008, ICONN 2008 ; Conference date: 25-02-2008 Through 29-02-2008",

}

Nasabi, M, Mitchell, A, Kalantar-Zadeh, K & Nesbitt, WS 2008, Microstamp patterning of protein arrays. in Proceedings of the 2008 International Conference on Nanoscience and Nanotechnology, ICONN 2008. IEEE, Institute of Electrical and Electronics Engineers, pp. 117-120, International Conference on Nanoscience and Nanotechnology (ICONN) 2008, Melbourne, Victoria, Australia, 25/02/08. https://doi.org/10.1109/ICONN.2008.4639260

Microstamp patterning of protein arrays. / Nasabi, Mahyar; Mitchell, Arnan; Kalantar-Zadeh, Kourosh et al.
Proceedings of the 2008 International Conference on Nanoscience and Nanotechnology, ICONN 2008. IEEE, Institute of Electrical and Electronics Engineers, 2008. p. 117-120.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Microstamp patterning of protein arrays

AU - Nasabi, Mahyar

AU - Mitchell, Arnan

AU - Kalantar-Zadeh, Kourosh

AU - Nesbitt, Warwick S.

N1 - Conference code: 2nd

PY - 2008/11/24

Y1 - 2008/11/24

N2 - Pathological thrombus formation is initiated by the interaction of blood platelets to immobilized proteins at the vessel wall. Platelet surface adhesion leads to biochemical activation and structural reorganization resulting in spreading of the platelet across the adhesive surface. Extensive studies have been carried out to examine platelet spreading responses on continuous substrates but little is known about the impact of protein surface distribution on platelet function. This paper describes the development of a micro-contact printing technique to establish defined 2-dimensional arrays of the thrombogenic protein fibrinogen at the surface of glass substrates, with the aim of investigating the impact of protein surface distribution on platelet biochemical signaling events associated with the adhesion/spreading process.

AB - Pathological thrombus formation is initiated by the interaction of blood platelets to immobilized proteins at the vessel wall. Platelet surface adhesion leads to biochemical activation and structural reorganization resulting in spreading of the platelet across the adhesive surface. Extensive studies have been carried out to examine platelet spreading responses on continuous substrates but little is known about the impact of protein surface distribution on platelet function. This paper describes the development of a micro-contact printing technique to establish defined 2-dimensional arrays of the thrombogenic protein fibrinogen at the surface of glass substrates, with the aim of investigating the impact of protein surface distribution on platelet biochemical signaling events associated with the adhesion/spreading process.

KW - Fibrinogen

KW - Microstamp

KW - PDMS

KW - Platelet

UR - https://www.scopus.com/pages/publications/56349133240

U2 - 10.1109/ICONN.2008.4639260

DO - 10.1109/ICONN.2008.4639260

M3 - Conference Paper

AN - SCOPUS:56349133240

SN - 1424415047

SN - 9781424415045

SP - 117

EP - 120

BT - Proceedings of the 2008 International Conference on Nanoscience and Nanotechnology, ICONN 2008

PB - IEEE, Institute of Electrical and Electronics Engineers

T2 - International Conference on Nanoscience and Nanotechnology (ICONN) 2008

Y2 - 25 February 2008 through 29 February 2008

ER -

Microstamp patterning of protein arrays

Abstract

Conference

Keywords

Access to Document

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