Microscopical investigations of nisin-loaded nanoliposomes prepared by Mozafari method and their bacterial targeting

Jean-Christophe Colas, Wanlong Shi, V SN Malleswara Rao, Abdel Wahab Omri, Mohammad Reza Mozafari, Harjinder Singh

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177 Citations (Scopus)


Nanoencapsulation may improve activity of protein or polypeptide antimicrobials against a variety of microorganisms. In this study, nanoliposomes prepared from different lipids (Phospholipon 90H, Phospholipon 100H, dipalmitoylphosphatidylcholine (DPPC), stearylamine (SA), dicetyl phosphate (DCP) and cholesterol) by a new, non-toxic and scalable method, were tested for their capacity to encapsulate nisin Z and target bacteria (Bacillus subtilis and Pseudomonasaeruginosa). Factors affecting the entrapment efficiency (charge and cholesterol concentration in the vesicles) and stability of nanoliposomes were assessed. The nanoliposomes and their bacterial targeting were visualised, using different microscopes under air and liquid environments. Nisin was entrapped in different nanoliposomes with encapsulation efficiencies (EE) ranging from 12 to 54 . Anionic vesicles possessed the highest EE for nisin while increase in cholesterol content in lipid membranes up to 20 molar ratio resulted in a reduction in EE. Stability of nanoliposome-encapsulated nisin was demonstrated for at least 14 months at 4 degrees C (DPPC:DCP:CHOL vesicles) and for 12 months at 25 degrees C (DPPC:SA:CHOL vesicles).
Original languageEnglish
Pages (from-to)841 - 847
Number of pages7
Issue number8
Publication statusPublished - 2007

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