MicroRNAs in hyperglycemia induced endothelial cell dysfunction

Maskomani Silambarasan, Jun Rong Tan, Dwi Setyowati Karolina, Arunmozhiarasi Armugam, Charanjit Kaur, Kandiah Jeyaseelan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Hyperglycemia is closely associated with prediabetes and Type 2 Diabetes Mellitus. Hyperglycemia increases the risk of vascular complications such as diabetic retinopathy, diabetic nephropathy, peripheral vascular disease and cerebro/cardiovascular diseases. Under hyperglycemic conditions, the endothelial cells become dysfunctional. In this study, we investigated the miRNA expression changes in human umbilical vein endothelial cells exposed to different glucose concentrations (5, 10, 25 and 40 mM glucose) and at various time intervals (6, 12, 24 and 48 h). miRNA microarray analyses showed that there is a correlation between hyperglycemia induced endothelial dysfunction and miRNA expression. In silico pathways analyses on the altered miRNA expression showed that the majority of the affected biological pathways appeared to be associated to endothelial cell dysfunction and apoptosis. We found the expression of ten miRNAs (miR-26a-5p, -26b-5p, 29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -140-5p, -192-5p, -221-3p and -320a) to increase gradually with increasing concentration of glucose. These miRNAs were also found to be involved in endothelial dysfunction. At least seven of them, miR-29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -221-3p, -320a and -192-5p, can be correlated to endothelial cell apoptosis.
Original languageEnglish
Number of pages22
JournalInternational Journal of Molecular Sciences
Volume17
Issue number4
DOIs
Publication statusPublished - 7 Apr 2016

Keywords

  • microRNA
  • diabetes mellitus
  • endothelial dysfunction
  • apoptosis
  • hyperglycemia

Cite this

Silambarasan, M., Tan, J. R., Karolina, D. S., Armugam, A., Kaur, C., & Jeyaseelan, K. (2016). MicroRNAs in hyperglycemia induced endothelial cell dysfunction. International Journal of Molecular Sciences, 17(4). https://doi.org/10.3390/ijms17040518
Silambarasan, Maskomani ; Tan, Jun Rong ; Karolina, Dwi Setyowati ; Armugam, Arunmozhiarasi ; Kaur, Charanjit ; Jeyaseelan, Kandiah. / MicroRNAs in hyperglycemia induced endothelial cell dysfunction. In: International Journal of Molecular Sciences. 2016 ; Vol. 17, No. 4.
@article{fed1cf9ad9bc40219ffaa554dd82fb6f,
title = "MicroRNAs in hyperglycemia induced endothelial cell dysfunction",
abstract = "Hyperglycemia is closely associated with prediabetes and Type 2 Diabetes Mellitus. Hyperglycemia increases the risk of vascular complications such as diabetic retinopathy, diabetic nephropathy, peripheral vascular disease and cerebro/cardiovascular diseases. Under hyperglycemic conditions, the endothelial cells become dysfunctional. In this study, we investigated the miRNA expression changes in human umbilical vein endothelial cells exposed to different glucose concentrations (5, 10, 25 and 40 mM glucose) and at various time intervals (6, 12, 24 and 48 h). miRNA microarray analyses showed that there is a correlation between hyperglycemia induced endothelial dysfunction and miRNA expression. In silico pathways analyses on the altered miRNA expression showed that the majority of the affected biological pathways appeared to be associated to endothelial cell dysfunction and apoptosis. We found the expression of ten miRNAs (miR-26a-5p, -26b-5p, 29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -140-5p, -192-5p, -221-3p and -320a) to increase gradually with increasing concentration of glucose. These miRNAs were also found to be involved in endothelial dysfunction. At least seven of them, miR-29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -221-3p, -320a and -192-5p, can be correlated to endothelial cell apoptosis.",
keywords = "microRNA, diabetes mellitus, endothelial dysfunction, apoptosis, hyperglycemia",
author = "Maskomani Silambarasan and Tan, {Jun Rong} and Karolina, {Dwi Setyowati} and Arunmozhiarasi Armugam and Charanjit Kaur and Kandiah Jeyaseelan",
year = "2016",
month = "4",
day = "7",
doi = "10.3390/ijms17040518",
language = "English",
volume = "17",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI AG",
number = "4",

}

Silambarasan, M, Tan, JR, Karolina, DS, Armugam, A, Kaur, C & Jeyaseelan, K 2016, 'MicroRNAs in hyperglycemia induced endothelial cell dysfunction' International Journal of Molecular Sciences, vol. 17, no. 4. https://doi.org/10.3390/ijms17040518

MicroRNAs in hyperglycemia induced endothelial cell dysfunction. / Silambarasan, Maskomani; Tan, Jun Rong; Karolina, Dwi Setyowati; Armugam, Arunmozhiarasi; Kaur, Charanjit; Jeyaseelan, Kandiah.

In: International Journal of Molecular Sciences, Vol. 17, No. 4, 07.04.2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - MicroRNAs in hyperglycemia induced endothelial cell dysfunction

AU - Silambarasan, Maskomani

AU - Tan, Jun Rong

AU - Karolina, Dwi Setyowati

AU - Armugam, Arunmozhiarasi

AU - Kaur, Charanjit

AU - Jeyaseelan, Kandiah

PY - 2016/4/7

Y1 - 2016/4/7

N2 - Hyperglycemia is closely associated with prediabetes and Type 2 Diabetes Mellitus. Hyperglycemia increases the risk of vascular complications such as diabetic retinopathy, diabetic nephropathy, peripheral vascular disease and cerebro/cardiovascular diseases. Under hyperglycemic conditions, the endothelial cells become dysfunctional. In this study, we investigated the miRNA expression changes in human umbilical vein endothelial cells exposed to different glucose concentrations (5, 10, 25 and 40 mM glucose) and at various time intervals (6, 12, 24 and 48 h). miRNA microarray analyses showed that there is a correlation between hyperglycemia induced endothelial dysfunction and miRNA expression. In silico pathways analyses on the altered miRNA expression showed that the majority of the affected biological pathways appeared to be associated to endothelial cell dysfunction and apoptosis. We found the expression of ten miRNAs (miR-26a-5p, -26b-5p, 29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -140-5p, -192-5p, -221-3p and -320a) to increase gradually with increasing concentration of glucose. These miRNAs were also found to be involved in endothelial dysfunction. At least seven of them, miR-29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -221-3p, -320a and -192-5p, can be correlated to endothelial cell apoptosis.

AB - Hyperglycemia is closely associated with prediabetes and Type 2 Diabetes Mellitus. Hyperglycemia increases the risk of vascular complications such as diabetic retinopathy, diabetic nephropathy, peripheral vascular disease and cerebro/cardiovascular diseases. Under hyperglycemic conditions, the endothelial cells become dysfunctional. In this study, we investigated the miRNA expression changes in human umbilical vein endothelial cells exposed to different glucose concentrations (5, 10, 25 and 40 mM glucose) and at various time intervals (6, 12, 24 and 48 h). miRNA microarray analyses showed that there is a correlation between hyperglycemia induced endothelial dysfunction and miRNA expression. In silico pathways analyses on the altered miRNA expression showed that the majority of the affected biological pathways appeared to be associated to endothelial cell dysfunction and apoptosis. We found the expression of ten miRNAs (miR-26a-5p, -26b-5p, 29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -140-5p, -192-5p, -221-3p and -320a) to increase gradually with increasing concentration of glucose. These miRNAs were also found to be involved in endothelial dysfunction. At least seven of them, miR-29b-3p, -29c-3p, -125b-1-3p, -130b-3p, -221-3p, -320a and -192-5p, can be correlated to endothelial cell apoptosis.

KW - microRNA

KW - diabetes mellitus

KW - endothelial dysfunction

KW - apoptosis

KW - hyperglycemia

UR - http://www.ncbi.nlm.nih.gov/pubmed/27070575

U2 - 10.3390/ijms17040518

DO - 10.3390/ijms17040518

M3 - Article

VL - 17

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 4

ER -