MicroRNAs-140-5p/140-3p modulate Leydig cell numbers in the developing mouse testis

Joanna Rakoczy, Selene L Fernandez-Valverde, Evgeny A Glazov, Elanor Wainwright, Tempei Sato, Shuji Takada, Alexander Combes, Darren J Korbie, David Miller, Sean M Grimmond, Melissa H Little, Hiroshi Asahara, John Stanley Mattick, Ryan J Taft, Dagmar Wilhelm

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MicroRNAs (miRNAs) have been shown to play key regulatory roles in a range of biological processes, including cell differentiation and development. To identify miRNAs that participate in gonad differentiation, a fundamental and tightly regulated developmental process, we examined miRNA expression profiles at the time of sex determination and during the early fetal differentiation of mouse testes and ovaries using high-throughput sequencing. We identified several miRNAs that were expressed in a sexually dimorphic pattern, including several members of the let-7 family, miR-378, and miR-140-3p. We focused our analysis on the most highly expressed, sexually dimorphic miRNA, miR-140-3p, and found that both miR-140-3p and its more lowly expressed counterpart, the previously annotated guide strand, miR-140-5p, are testis enriched and expressed in testis cords. Analysis of the miR-140-5p/miR-140-3p-null mouse revealed a significant increase in the number of Leydig cells in the developing XY gonad, strongly suggesting an important role for miR-140-5p/miR-140-3p in testis differentiation in mouse.
Original languageEnglish
Article number143
Number of pages11
JournalBiology of Reproduction
Issue number6
Publication statusPublished - 2013

Cite this

Rakoczy, J., Fernandez-Valverde, S. L., Glazov, E. A., Wainwright, E., Sato, T., Takada, S., Combes, A., Korbie, D. J., Miller, D., Grimmond, S. M., Little, M. H., Asahara, H., Mattick, J. S., Taft, R. J., & Wilhelm, D. (2013). MicroRNAs-140-5p/140-3p modulate Leydig cell numbers in the developing mouse testis. Biology of Reproduction, 88(6 ), [143]. https://doi.org/10.1095/biolreprod.113.107607