microRNA expression in blood of dengue patients

Paul A Tambyah, Chai S Ching, Sugunavathi Sepramaniam, Jaminah M Ali, Arunmozhiarasi Armugam, Kandiah Jeyaseelan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND: Dengue is the most common arboviral illness worldwide. While most infected patients recover, a proportion of them develop severe complications or fatality. Nevertheless, the pathophysiological mechanisms which distinguish the disease severity and associated complications are not clearly understood. We studied blood profiles of dengue patients in order to identify microRNAs that could play a role in these pathophysiological mechanisms. METHODS: Blood samples from 26 dengue-infected patients were collected within 0-14 days of infection. Together with samples obtained from six healthy individuals, microRNA profiles were generated to identify significantly altered microRNAs upon dengue infection. Profiles of patients with influenza were also used to determine the disease specificity of these altered microRNAs. Their discriminative power to distinguish dengue from influenza was then tested statistically. RESULTS: Several significantly altered microRNAs were identified in patients with dengue. Twelve microRNAs were specifically altered upon acute dengue whereas 14 microRNAs exhibited similar expression between dengue and influenza. Seventeen microRNAs which could potentially distinguish dengue-related complications were also identified. Expression of miR-24-1-5p, miR-512-5p and miR-4640-3p distinguished mild dengue from those exhibiting liver complications whereas miR-383 was significantly upregulated in mild dengue compared to those diagnosed as severe dengue with fluid accumulation. CONCLUSIONS: We identified two panels of microRNAs - one specific for dengue and the other common to dengue and influenza. We also report on the differentially expressed microRNAs in patients with mild versus severe dengue, which could be the basis for the complications seen in them.
Original languageEnglish
Pages (from-to)466-476
Number of pages11
JournalAnnals of Clinical Biochemistry
Volume53
Issue number4
DOIs
Publication statusPublished - Jul 2016

Keywords

  • clinical studies
  • DNA and RNA techniques
  • genetics
  • laboratory methods

Cite this

Tambyah, P. A., Ching, C. S., Sepramaniam, S., Ali, J. M., Armugam, A., & Jeyaseelan, K. (2016). microRNA expression in blood of dengue patients. Annals of Clinical Biochemistry, 53(4), 466-476. https://doi.org/10.1177/0004563215604001
Tambyah, Paul A ; Ching, Chai S ; Sepramaniam, Sugunavathi ; Ali, Jaminah M ; Armugam, Arunmozhiarasi ; Jeyaseelan, Kandiah. / microRNA expression in blood of dengue patients. In: Annals of Clinical Biochemistry. 2016 ; Vol. 53, No. 4. pp. 466-476.
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Tambyah, PA, Ching, CS, Sepramaniam, S, Ali, JM, Armugam, A & Jeyaseelan, K 2016, 'microRNA expression in blood of dengue patients' Annals of Clinical Biochemistry, vol. 53, no. 4, pp. 466-476. https://doi.org/10.1177/0004563215604001

microRNA expression in blood of dengue patients. / Tambyah, Paul A; Ching, Chai S; Sepramaniam, Sugunavathi; Ali, Jaminah M; Armugam, Arunmozhiarasi; Jeyaseelan, Kandiah.

In: Annals of Clinical Biochemistry, Vol. 53, No. 4, 07.2016, p. 466-476.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - microRNA expression in blood of dengue patients

AU - Tambyah, Paul A

AU - Ching, Chai S

AU - Sepramaniam, Sugunavathi

AU - Ali, Jaminah M

AU - Armugam, Arunmozhiarasi

AU - Jeyaseelan, Kandiah

PY - 2016/7

Y1 - 2016/7

N2 - BACKGROUND: Dengue is the most common arboviral illness worldwide. While most infected patients recover, a proportion of them develop severe complications or fatality. Nevertheless, the pathophysiological mechanisms which distinguish the disease severity and associated complications are not clearly understood. We studied blood profiles of dengue patients in order to identify microRNAs that could play a role in these pathophysiological mechanisms. METHODS: Blood samples from 26 dengue-infected patients were collected within 0-14 days of infection. Together with samples obtained from six healthy individuals, microRNA profiles were generated to identify significantly altered microRNAs upon dengue infection. Profiles of patients with influenza were also used to determine the disease specificity of these altered microRNAs. Their discriminative power to distinguish dengue from influenza was then tested statistically. RESULTS: Several significantly altered microRNAs were identified in patients with dengue. Twelve microRNAs were specifically altered upon acute dengue whereas 14 microRNAs exhibited similar expression between dengue and influenza. Seventeen microRNAs which could potentially distinguish dengue-related complications were also identified. Expression of miR-24-1-5p, miR-512-5p and miR-4640-3p distinguished mild dengue from those exhibiting liver complications whereas miR-383 was significantly upregulated in mild dengue compared to those diagnosed as severe dengue with fluid accumulation. CONCLUSIONS: We identified two panels of microRNAs - one specific for dengue and the other common to dengue and influenza. We also report on the differentially expressed microRNAs in patients with mild versus severe dengue, which could be the basis for the complications seen in them.

AB - BACKGROUND: Dengue is the most common arboviral illness worldwide. While most infected patients recover, a proportion of them develop severe complications or fatality. Nevertheless, the pathophysiological mechanisms which distinguish the disease severity and associated complications are not clearly understood. We studied blood profiles of dengue patients in order to identify microRNAs that could play a role in these pathophysiological mechanisms. METHODS: Blood samples from 26 dengue-infected patients were collected within 0-14 days of infection. Together with samples obtained from six healthy individuals, microRNA profiles were generated to identify significantly altered microRNAs upon dengue infection. Profiles of patients with influenza were also used to determine the disease specificity of these altered microRNAs. Their discriminative power to distinguish dengue from influenza was then tested statistically. RESULTS: Several significantly altered microRNAs were identified in patients with dengue. Twelve microRNAs were specifically altered upon acute dengue whereas 14 microRNAs exhibited similar expression between dengue and influenza. Seventeen microRNAs which could potentially distinguish dengue-related complications were also identified. Expression of miR-24-1-5p, miR-512-5p and miR-4640-3p distinguished mild dengue from those exhibiting liver complications whereas miR-383 was significantly upregulated in mild dengue compared to those diagnosed as severe dengue with fluid accumulation. CONCLUSIONS: We identified two panels of microRNAs - one specific for dengue and the other common to dengue and influenza. We also report on the differentially expressed microRNAs in patients with mild versus severe dengue, which could be the basis for the complications seen in them.

KW - clinical studies

KW - DNA and RNA techniques

KW - genetics

KW - laboratory methods

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DO - 10.1177/0004563215604001

M3 - Article

VL - 53

SP - 466

EP - 476

JO - Annals of Clinical Biochemistry

JF - Annals of Clinical Biochemistry

SN - 0004-5632

IS - 4

ER -

Tambyah PA, Ching CS, Sepramaniam S, Ali JM, Armugam A, Jeyaseelan K. microRNA expression in blood of dengue patients. Annals of Clinical Biochemistry. 2016 Jul;53(4):466-476. https://doi.org/10.1177/0004563215604001