MicroRNA-100 regulates osteogenic differentiation of human adipose-derived mesenchymal stem cells by targeting BMPR2

Yang Zeng, Xuebin Qu, Hongling Li, Shan Huang, Shihua Wang, Qilin Xu, Ruizhu Lin, Qin Han, Jing Li, Robert Chunhua Zhao

Research output: Contribution to journalArticleResearchpeer-review

126 Citations (Scopus)

Abstract

Elucidation of the molecular mechanisms governing human adipose-derived mesenchymal stem cells (hASCs) osteogenic differentiation is of great importance for improving the treatment of bone-related diseases. In this study, we examined the role of microRNA (miR)-100 on the osteogenesis of hASCs. Overexpression of miR-100 inhibited osteogenic differentiation of hASCs in vitro, whereas downregulation of miR-100 enhanced the process. Target prediction analysis and dual luciferase report assay confirmed that bone morphogenetic protein receptor type II (BMPR2) was a direct target of miR-100. Furthermore, knockdown of BMPR2 by RNA interference inhibited osteogenic differentiation of hASCs, similar as the effect of upregulation miR-100. Taken together, our findings imply that miR-100 plays a negative role in osteogenic differentiation and might act through targeting BMPR2. Crown

Original languageEnglish
Pages (from-to)2375-2381
Number of pages7
JournalFEBS Letters
Volume586
Issue number16
DOIs
Publication statusPublished - 30 Jul 2012
Externally publishedYes

Keywords

  • BMPR2
  • hASCs
  • miR-100
  • Osteogenesis

Cite this