TY - JOUR
T1 - Micropreparative ion exchange high performance liquid chromatography
T2 - Applications to microsequence analysis
AU - Nice, E. C.
AU - Fabri, L.
AU - Hammacher, A.
AU - Andersson, K.
AU - Hellman, U.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Data on the characterization of anion and cation micropreparative (50X1.6 mm i.d.) HPLC columns is presented. It is shown how subnanomole quantities of protein can be efficiently recovered from such columns, rendering them compatible for use in multidimensional chromatographic strategies for the purification of trace biological samples. By selection of appropriate solvent systems (e.g., buffer‐free sodium chloride solutions), the small eluant peak volumes can be loaded directly onto the gas phase sequencer, and N‐terminal sequence data obtained. The potential of the technique is illustrated for the purification of a GTPase activating protein (GAP‐3).
AB - Data on the characterization of anion and cation micropreparative (50X1.6 mm i.d.) HPLC columns is presented. It is shown how subnanomole quantities of protein can be efficiently recovered from such columns, rendering them compatible for use in multidimensional chromatographic strategies for the purification of trace biological samples. By selection of appropriate solvent systems (e.g., buffer‐free sodium chloride solutions), the small eluant peak volumes can be loaded directly onto the gas phase sequencer, and N‐terminal sequence data obtained. The potential of the technique is illustrated for the purification of a GTPase activating protein (GAP‐3).
UR - http://www.scopus.com/inward/record.url?scp=0027288740&partnerID=8YFLogxK
U2 - 10.1002/bmc.1130070212
DO - 10.1002/bmc.1130070212
M3 - Article
C2 - 8485372
AN - SCOPUS:0027288740
VL - 7
SP - 104
EP - 111
JO - Biomedical Chromatography
JF - Biomedical Chromatography
SN - 0269-3879
IS - 2
ER -