Micropreparative high performance liquid chromatography of proteins and peptides: Principles and applications

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Abstract

The role of micropreparative high performance liquid chromatography columns for the efficient purification of microgram to nanogram quantities of proteins and peptides in small eluent volumes at high concentration is described. The historical development of the technique with respect to column dimensions and instrumentation is outlined. The potential of this technique to prepare samples for use with highly specific and sensitive analytical techniques (e.g., N-terminal sequence analysis, mass specirometry. biosensor analysis, biological assay) or for the recovery of low levels of material following chemical modification (e.g., radio iodination, enzymatic semisynthesis, chemical conjugatioin) is illustrated for a number of proteins including growth factors and their receptors.

Original languageEnglish
Pages (from-to)319-341
Number of pages23
JournalBiopolymers (Peptide Science)
Volume40
Issue number3
Publication statusPublished - 1 Dec 1996
Externally publishedYes

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