Abstract
Understanding the effect of liposome size on tendency for accumulation in tumour tissue requires preparation of defined populations of different sized particles. However, controlling the size distributions without changing the lipid composition is difficult, and differences in compositions itself modify distribution behaviour. Here, a commercial microfluidic format as well as traditional methods was used to prepare doxorubicin-loaded liposomes of different size distributions but with the same lipid composition, and drug retention, biodistribution and localization in tumour tissues were evaluated. The small (∼50 nm diameter) liposomes prepared by microfluidics and large (∼75 nm diameter) liposomes displayed similar drug retention in in vitro release studies, and similar biodistribution patterns in tumour-bearing mice. However, the extent of extravasation was clearly dependent on size of the liposomes, with the small liposomes showing tissue distribution beyond the vascular area compared to the large liposomes. The use of microfluidics to prepare smaller size distribution liposomes compared to sonication methods is demonstrated, and allowed preparation of different size distribution drug carriers from the same lipid composition to enable new understanding of tissue distribution in compositionally consistent materials is demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Journal of Liposome Research |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 |
Keywords
- biodistribution
- Liposome
- microfluidics
- size distribution
- tumour penetration
Cite this
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Microfluidic preparation of drug-loaded PEGylated liposomes, and the impact of liposome size on tumour retention and penetration. / Dong, Yao Da; Tchung, Estefania; Nowell, Cameron; Kaga, Sadik; Leong, Nathania; Mehta, Dharmini; Kaminskas, Lisa M.; Boyd, Ben J.
In: Journal of Liposome Research, Vol. 29, No. 1, 2019, p. 1-9.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Microfluidic preparation of drug-loaded PEGylated liposomes, and the impact of liposome size on tumour retention and penetration
AU - Dong, Yao Da
AU - Tchung, Estefania
AU - Nowell, Cameron
AU - Kaga, Sadik
AU - Leong, Nathania
AU - Mehta, Dharmini
AU - Kaminskas, Lisa M.
AU - Boyd, Ben J.
PY - 2019
Y1 - 2019
N2 - Understanding the effect of liposome size on tendency for accumulation in tumour tissue requires preparation of defined populations of different sized particles. However, controlling the size distributions without changing the lipid composition is difficult, and differences in compositions itself modify distribution behaviour. Here, a commercial microfluidic format as well as traditional methods was used to prepare doxorubicin-loaded liposomes of different size distributions but with the same lipid composition, and drug retention, biodistribution and localization in tumour tissues were evaluated. The small (∼50 nm diameter) liposomes prepared by microfluidics and large (∼75 nm diameter) liposomes displayed similar drug retention in in vitro release studies, and similar biodistribution patterns in tumour-bearing mice. However, the extent of extravasation was clearly dependent on size of the liposomes, with the small liposomes showing tissue distribution beyond the vascular area compared to the large liposomes. The use of microfluidics to prepare smaller size distribution liposomes compared to sonication methods is demonstrated, and allowed preparation of different size distribution drug carriers from the same lipid composition to enable new understanding of tissue distribution in compositionally consistent materials is demonstrated.
AB - Understanding the effect of liposome size on tendency for accumulation in tumour tissue requires preparation of defined populations of different sized particles. However, controlling the size distributions without changing the lipid composition is difficult, and differences in compositions itself modify distribution behaviour. Here, a commercial microfluidic format as well as traditional methods was used to prepare doxorubicin-loaded liposomes of different size distributions but with the same lipid composition, and drug retention, biodistribution and localization in tumour tissues were evaluated. The small (∼50 nm diameter) liposomes prepared by microfluidics and large (∼75 nm diameter) liposomes displayed similar drug retention in in vitro release studies, and similar biodistribution patterns in tumour-bearing mice. However, the extent of extravasation was clearly dependent on size of the liposomes, with the small liposomes showing tissue distribution beyond the vascular area compared to the large liposomes. The use of microfluidics to prepare smaller size distribution liposomes compared to sonication methods is demonstrated, and allowed preparation of different size distribution drug carriers from the same lipid composition to enable new understanding of tissue distribution in compositionally consistent materials is demonstrated.
KW - biodistribution
KW - Liposome
KW - microfluidics
KW - size distribution
KW - tumour penetration
UR - http://www.scopus.com/inward/record.url?scp=85041295600&partnerID=8YFLogxK
U2 - 10.1080/08982104.2017.1391285
DO - 10.1080/08982104.2017.1391285
M3 - Article
VL - 29
SP - 1
EP - 9
JO - Journal of Liposome Research
JF - Journal of Liposome Research
SN - 0898-2104
IS - 1
ER -