Microfluidic platform for separation and extraction of plasma from whole blood using dielectrophoresis

Crispin Szydzik, Khashayar Khoshmanesh, Arnan Mitchell, Christian Karnutsch

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

Microfluidic based blood plasma extraction is a fundamental necessity that will facilitate many future lab-on-a-chip based point-of-care diagnostic systems. However, current approaches for providing this analyte are hampered by the requirement to provide external pumping or dilution of blood, which result in low effective yield, lower concentration of target constituents, and complicated functionality. This paper presents a capillary-driven, dielectrophoresis-enabled microfluidic system capable of separating and extracting cell-free plasma from small amounts of whole human blood. This process takes place directly on-chip, and without the requirement of dilution, thus eliminating the prerequisite of preprocessed blood samples and external liquid handling systems. The microfluidic chip takes advantage of a capillary pump for driving whole blood through the main channel and a cross flow filtration system for extracting plasma from whole blood. This filter is actively unblocked through negative dielectrophoresis forces, dramatically enhancing the volume of extracted plasma. Experiments using whole human blood yield volumes of around 180 nl of cell-free, undiluted plasma. We believe that implementation of various integrated biosensing techniques into this plasma extraction system could enable multiplexed detection of various biomarkers.

Original languageEnglish
Article number064120
Pages (from-to)1-16
Number of pages16
JournalBiomicrofluidics
Volume9
Issue number6
DOIs
Publication statusPublished - 1 Nov 2015
Externally publishedYes

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