TY - JOUR
T1 - Microcin 7
T2 - Purification and properties
AU - García-Bustos, J. F.
AU - Pezzi, N.
AU - Asensio, C.
PY - 1984/3/15
Y1 - 1984/3/15
N2 - Microcin 7 is an antibiotic peptide, produced and excreted to the culture medium by E. coli strains harboring the plasmid pRYC7. This peptide was extracted from the culture media by adsorbing it on octadecyl silica. It was purified by gel filtration on Sephadex G-25 and reverse phase high performance liquid chromatography. Its amino acid composition is the following: Ala (0.8), Arg (1.9), Asx (1.9), Gly (1.5), Met (0.8) and Thr (0.9). The purified peptide does not react with ninhydrin and it is resistant to carboxypeptidase degradation, indicating that the molecule may be a cyclic or end-blocked oligopeptide.
AB - Microcin 7 is an antibiotic peptide, produced and excreted to the culture medium by E. coli strains harboring the plasmid pRYC7. This peptide was extracted from the culture media by adsorbing it on octadecyl silica. It was purified by gel filtration on Sephadex G-25 and reverse phase high performance liquid chromatography. Its amino acid composition is the following: Ala (0.8), Arg (1.9), Asx (1.9), Gly (1.5), Met (0.8) and Thr (0.9). The purified peptide does not react with ninhydrin and it is resistant to carboxypeptidase degradation, indicating that the molecule may be a cyclic or end-blocked oligopeptide.
UR - http://www.scopus.com/inward/record.url?scp=0021763048&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(84)80318-6
DO - 10.1016/S0006-291X(84)80318-6
M3 - Article
C2 - 6370248
AN - SCOPUS:0021763048
VL - 119
SP - 779
EP - 785
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -