Microbial-type terpene synthase genes occur widely in nonseed land plants, but not in seed plants

Qidong Jia, Guanglin Li, Tobias G. Köllner, Jianyu Fu, Xinlu Chen, Wangdan Xiong, Barbara J. Crandall-Stotler, John Bowman, David J. Weston, Yong Zhang, Li Chen, Yinlong Xie, Fay Wei Li, Carl J Rothfels, Anders Larsson, Sean W Graham, Dennis W. Stevenson, Gane Ka-Shu Wong, Jonathan Gershenzon, Feng Chen

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30 Citations (Scopus)

Abstract

The vast abundance of terpene natural products in nature is due to enzymes known as terpene synthases (TPSs) that convert acyclic prenyl diphosphate precursors into a multitude of cyclic and acyclic carbon skeletons. Yet the evolution of TPSs is not well understood at higher levels of classification. Microbial TPSs from bacteria and fungi are only distantly related to typical plant TPSs, whereas genes similar to microbial TPS genes have been recently identified in the lycophyte Selaginella moellendorffii. The goal of this study was to investigate the distribution, evolution, and biochemical functions of microbial terpene synthase-like (MTPSL) genes in other plants. By analyzing the transcriptomes of 1,103 plant species ranging from green algae to flowering plants, putative MTPSL genes were identified predominantly from nonseed plants, including liverworts, mosses, hornworts, lycophytes, and monilophytes. Directed searching for MTPSL genes in the sequenced genomes of a wide range of seed plants confirmed their general absence in this group. Among themselves, MTPSL proteins from nonseed plants form four major groups, with two of these more closely related to bacterial TPSs and the other two to fungal TPSs. Two of the four groups contain a canonical aspartate-rich "DDxxD" motif. The third group has a "DDxxxD" motif, and the fourth group has only the first two "DD" conserved in this motif. Upon heterologous expression, representative members from each of the four groups displayed diverse catalytic functions as monoterpene and sesquiterpene synthases, suggesting these are important for terpene formation in nonseed plants.

Original languageEnglish
Pages (from-to)12328-12333
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number43
DOIs
Publication statusPublished - 25 Oct 2016

Keywords

  • Gene evolution
  • Nonseed plant
  • Specialized metabolism
  • Terpene synthase

Cite this

Jia, Q., Li, G., Köllner, T. G., Fu, J., Chen, X., Xiong, W., Crandall-Stotler, B. J., Bowman, J., Weston, D. J., Zhang, Y., Chen, L., Xie, Y., Li, F. W., Rothfels, C. J., Larsson, A., Graham, S. W., Stevenson, D. W., Wong, G. K-S., Gershenzon, J., & Chen, F. (2016). Microbial-type terpene synthase genes occur widely in nonseed land plants, but not in seed plants. Proceedings of the National Academy of Sciences of the United States of America, 113(43), 12328-12333. https://doi.org/10.1073/pnas.1607973113