Microarrays for the evaluation of cell-biomaterial surface interactions

H. Thissen, G. Johnson, G. McFarland, B. C H Verbiest, T. Gengenbach, N. H. Voelcker

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

7 Citations (Scopus)

Abstract

The evaluation of cell-material surface interactions is important for the design of novel biomaterials which are used in a variety of biomedical applications. While traditional in vitro test methods have routinely used samples of relatively large size, microarrays representing different biomaterials offer many advantages, including high throughput and reduced sample handling. Here, we describe the simultaneous cell-based testing of matrices of polymeric biomaterials, arrayed on glass slides with a low cell-attachment background coating. Arrays were constructed using a microarray robot at 6 fold redundancy with solid pins having a diameter of 375 μm. Printed solutions contained at least one monomer, an initiator and a bifunctional crosslinker. After subsequent UV polymerisation, the arrays were washed and characterised by X-ray photoelectron spectroscopy. Cell culture experiments were carried out over 24 hours using HeLa cells. After labelling with CellTracker® Green for the final hour of incubation and subsequent fixation, the arrays were scanned. In addition, individual spots were also viewed by fluorescence microscopy. The evaluation of cell-surface interactions in high-throughput assays as demonstrated here is a key enabling technology for the effective development of future biomaterials.

Original languageEnglish
Title of host publicationSmart Materials IV
DOIs
Publication statusPublished - 27 Apr 2007
Externally publishedYes
EventSPIE International Symposium on Smart Materials, Nano- and Micro-Smart Systems - Adelaide, Australia
Duration: 11 Dec 200613 Dec 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6413
ISSN (Print)0277-786X

Conference

ConferenceSPIE International Symposium on Smart Materials, Nano- and Micro-Smart Systems
CountryAustralia
CityAdelaide
Period11/12/0613/12/06

Keywords

  • Biomaterials
  • Cell culture
  • High-throughput
  • In vitro
  • Microarrays

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