Microarray technology for mutation analysis of low-template DNA samples

Chelsea Loree Salvado, David Stephen Cram

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

    5 Citations (Scopus)

    Abstract

    Microarrays containing oligonucleotide mutation probes are emerging as useful platforms for the diagnosis of genetic disease. Herein, we describe the development and validation of an in-house microarray suitable for the diagnosis of common cystic fibrosis (CF) mutations in low-template DNA samples such as those taken for preimplantation genetic diagnosis and prenatal diagnosis. The success of the CF microarray was based on the ability to generate sufficient target DNA for hybridization to the array probes using either direct polymerase chain reaction (PCR) amplification or whole-genome amplification followed by PCR. From replicate experiments using target DNA carrying known CF mutations, it was possible to define strict diagnostic parameters for the accurate diagnosis of CF. This protocol serves as a general guide for DNA-testing laboratories to develop other microarray platforms that may eventually replace traditional PCR-based genetic testing in the near future.
    Original languageEnglish
    Title of host publicationSingle Cell Diagnostics: Methods and Protocols
    EditorsAlan Thornhill
    Place of PublicationTotowa NJ USA
    PublisherHumana Press
    Pages153 - 173
    Number of pages21
    Edition1st
    ISBN (Print)9781597452984
    Publication statusPublished - 2007

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