MICA exon 5 microsatellite typing by DNA heteroduplex analysis: A new polymorphism in the transmembrane region

L. Ricci Vitiani, I. Potolicchio, M. D'Amato, O. R. Baricordi, R. Sorrentino

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25 Citations (Scopus)

Abstract

MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.

Original languageEnglish
Pages (from-to)309-311
Number of pages3
JournalTissue Antigens
Volume51
Issue number3
Publication statusPublished - 25 Mar 1998
Externally publishedYes

Keywords

  • Heteroduplex
  • MICA (MHC class I chain-related gene A)
  • Polymorphism
  • STR (short tandem repeats)

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