MICA exon 5 microsatellite typing by DNA heteroduplex analysis: A new polymorphism in the transmembrane region

L. Ricci Vitiani; I. Potolicchio; M. D'Amato; O. R. Baricordi; R. Sorrentino

MICA exon 5 microsatellite typing by DNA heteroduplex analysis: A new polymorphism in the transmembrane region

L. Ricci Vitiani, I. Potolicchio, M. D'Amato, O. R. Baricordi, R. Sorrentino

Research output: Contribution to journal › Article › Research › peer-review

Abstract

MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.

Original language	English
Pages (from-to)	309-311
Number of pages	3
Journal	Tissue Antigens
Volume	51
Issue number	3
Publication status	Published - 25 Mar 1998
Externally published	Yes

Keywords

Heteroduplex
MICA (MHC class I chain-related gene A)
Polymorphism
STR (short tandem repeats)

Cite this

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title = "MICA exon 5 microsatellite typing by DNA heteroduplex analysis: A new polymorphism in the transmembrane region",

abstract = "MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.",

keywords = "Heteroduplex, MICA (MHC class I chain-related gene A), Polymorphism, STR (short tandem repeats)",

author = "Vitiani, {L. Ricci} and I. Potolicchio and M. D'Amato and Baricordi, {O. R.} and R. Sorrentino",

year = "1998",

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TY - JOUR

T1 - MICA exon 5 microsatellite typing by DNA heteroduplex analysis

T2 - A new polymorphism in the transmembrane region

AU - Vitiani, L. Ricci

AU - Potolicchio, I.

AU - D'Amato, M.

AU - Baricordi, O. R.

AU - Sorrentino, R.

PY - 1998/3/25

Y1 - 1998/3/25

N2 - MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.

AB - MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.

KW - Heteroduplex

KW - MICA (MHC class I chain-related gene A)

KW - Polymorphism

KW - STR (short tandem repeats)

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M3 - Article

C2 - 9550334

AN - SCOPUS:0031914472

SN - 0001-2815

VL - 51

SP - 309

EP - 311

JO - Tissue Antigens

JF - Tissue Antigens

IS - 3

ER -

MICA exon 5 microsatellite typing by DNA heteroduplex analysis: A new polymorphism in the transmembrane region

Abstract

Keywords

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