MHC class I-restricted cytotoxic lymphocyte responses induced by enterotoxin-based mucosal adjuvants

Cameron P. Simmons, Pietro Mastroeni, Ray Fowler, Marjan Ghaem-Maghami, Nils Lycke, Mariagrazia Pizza, Rino Rappuoli, Gordon Dougan

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Abstract

The ability of enterotoxin-based mucosal adjuvants to induce CD8+ MHC class I-restricted CTL responses to a codelivered bystander Ag was examined. Escherichia coli heat-labile toxin (LT), or derivatives of LT carrying mutations in the A subunit (LTR72, LTK63), were tested in parallel with cholera toxin (CT) or a fusion protein consisting of the A1 subunit of CT fused to the Ig binding domain of Staphylococcus aureus protein A (called CTA1-DD). Intranasal (i.n.) immunization of C57BL/6 mice with CT, CTA1-DD, LT, LTR72, LTK63, but not rLT-B, elicited MHC class I-restricted CD8+ T cell responses to coadministered OVA or the OVA CTL peptide SIINFEKL (OVA257- 264), CT, LT, and LTR72 also induced CTL responses to OVA after s.c. or oral coimmunization whereas LTK63 only activated responses after s.c. coimmunization. rLT-B was unable to adjuvant CTL responses to OVA or OVA257-264 administered by any route. Mice treated with an anti-CD4 mAb to deplete CD4+ T cells mounted significant OVA-specific CTL responses after i.n. coadministration of LT with OVA or OVA257-264. Both 51Cr release assays and IFN-γ enzyme-linked immunospot assays indicated that IFN- γ(-/-) and IL-12 p40(-/-) gene knockout mice developed CTL responses equivalent to those detected in normal C57BL/6 mice. The results highlight the versatility of toxin-based adjuvants and suggest that LT potentiates CTL responses independently of IL-12 and IFN-γ and probably by a mechanism unrelated to cross-priming.

Original languageEnglish
Pages (from-to)6502-6510
Number of pages9
JournalJournal of Immunology
Volume163
Issue number12
Publication statusPublished - 28 Dec 1999
Externally publishedYes

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