TY - JOUR
T1 - Mft52, an acid-bristle protein in the cytosol that delivers precursor proteins to yeast mitochondria
AU - Cartwright, Peter
AU - Beilharz, Traude
AU - Hansen, Per
AU - Garrett, Jinnie
AU - Lithgow, Trevor
PY - 1997/2/21
Y1 - 1997/2/21
N2 - We have identified a novel protein, Mft52, in the cytosol of yeast cells. Mft52 has a two-domain structure that includes a receptor-like carboxyl-terminal 'acid-bristle' domain, which binds basic, amphipathic mitochondrial targeting sequences. Native Mft52, purified from the cytosol of yeast cells, is found as a large particle eluting in the void volume of a Superose 6 gel filtration column. Fusion proteins, consisting of mitochondrial targeting sequences fused to nonmitochondrial passenger proteins, are targeted to mitochondria in wild-type yeast cells, but defects in the gene encoding Mft52 drastically reduce the delivery of these proteins to the mitochondria. We propose that Mft52 is a subunit of a particle that is part of a system of targeting factors and molecular chaperones mediating the earliest stages of protein targeting to the mitochondria.
AB - We have identified a novel protein, Mft52, in the cytosol of yeast cells. Mft52 has a two-domain structure that includes a receptor-like carboxyl-terminal 'acid-bristle' domain, which binds basic, amphipathic mitochondrial targeting sequences. Native Mft52, purified from the cytosol of yeast cells, is found as a large particle eluting in the void volume of a Superose 6 gel filtration column. Fusion proteins, consisting of mitochondrial targeting sequences fused to nonmitochondrial passenger proteins, are targeted to mitochondria in wild-type yeast cells, but defects in the gene encoding Mft52 drastically reduce the delivery of these proteins to the mitochondria. We propose that Mft52 is a subunit of a particle that is part of a system of targeting factors and molecular chaperones mediating the earliest stages of protein targeting to the mitochondria.
UR - http://www.scopus.com/inward/record.url?scp=0031053909&partnerID=8YFLogxK
U2 - 10.1074/jbc.272.8.5320
DO - 10.1074/jbc.272.8.5320
M3 - Article
C2 - 9030606
AN - SCOPUS:0031053909
SN - 0021-9258
VL - 272
SP - 5320
EP - 5325
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -