Methods to investigate seizures and associated cognitive decline using zebrafish model

Brandon Kar Meng Choo, Mohd Farooq Shaikh

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

    Abstract

    Epileptic seizures are the manifestation of several signs and/or symptoms due to heightened brain activity, causing a variety of disturbances such as motor and cognition dysfunction. In contrast, epilepsy is characterized by a chronic tendency to spawn these epileptic seizures. One way of studying epilepsy or seizures is to induce either acute seizures or epilepsy in animals via chemoconvulsants such as pentylenetetrazol (acute seizures) or kainic acid (chronic seizures), though pentylenetetrazol can also produce chronic seizures via kindling. Zebrafish are of great utility as an animal model as they have a high breeding rate and are genetically similar to humans. By intraperitoneally injecting chemoconvulsants into zebrafish, they develop seizure-like behavior, which can be scored to determine severity. By recording this behavior, the resulting locomotion pattern and parameters can also be tracked via software analysis. To investigate the cognitive decline comorbidity of epileptic seizures, mazes such as the T-maze and three-axis maze can be used to evaluate the learning and memory ability of the zebrafish via operant conditioning. This is done by comparing and contrasting the time taken for a zebrafish to reach the location of a reward over successive trials.

    Original languageEnglish
    Title of host publicationExperimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy
    EditorsDivya Vohora
    Place of PublicationUnited States
    PublisherHumana Press
    Chapter12
    Pages221-232
    Number of pages12
    ISBN (Electronic)9781071612545
    ISBN (Print)9781071612538
    DOIs
    Publication statusPublished - 2021

    Publication series

    NameNeuromethods
    Volume167
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045

    Keywords

    • Cognitive decline
    • Epilepsy
    • Kindling
    • Seizures
    • T-maze
    • Three-axis maze
    • Zebrafish (Danio rerio)

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