Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium

Jochen Grassinger; Susan Nilsson

doi:10.1007/978-1-61779-145-1

Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium

Jochen Grassinger, Susan Nilsson

Anatomy & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other

11 Citations (Scopus)

Abstract

The tracking of immunofluorescent labeled hematopoietic stem and progenitor cells (HSC/HPC) within the bone marrow (BM) cavity allows the assessment of the regulatory processes involved in transendothelial migration, trans-marrow migration, and finally lodgement into the HSC niche. This is of interest as the extracellular and cellular components involved in the regulation of HSC quiescence and differentiation are still not completely understood. Homing of transplanted HSC is the first critical step in the interaction between HSC and the microenvironment of the BM. As a consequence, murine models allowing the evaluation of the structural relationship between migrating HSC, the endosteal bone surface, and the vascular components of the BM enhance our understanding of hematopoietic regulation.

Original language	English
Title of host publication	Stem Cell Migration - Methods and Protocols
Editors	M-D Filippi, H Geiger
Place of Publication	USA
Publisher	Humana Press
Pages	197 - 214
Number of pages	18
Edition	1st
ISBN (Print)	978-1-61779-144-4
DOIs	https://doi.org/10.1007/978-1-61779-145-1
Publication status	Published - 2011

Access to Document

10.1007/978-1-61779-145-1

Cite this

Grassinger, J., & Nilsson, S. (2011). Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium. In M-D. Filippi, & H. Geiger (Eds.), Stem Cell Migration - Methods and Protocols (1st ed., pp. 197 - 214). Humana Press. https://doi.org/10.1007/978-1-61779-145-1

@inbook{5b45b2d871124088912ef4bc9663b3c4,

title = "Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium",

abstract = "The tracking of immunofluorescent labeled hematopoietic stem and progenitor cells (HSC/HPC) within the bone marrow (BM) cavity allows the assessment of the regulatory processes involved in transendothelial migration, trans-marrow migration, and finally lodgement into the HSC niche. This is of interest as the extracellular and cellular components involved in the regulation of HSC quiescence and differentiation are still not completely understood. Homing of transplanted HSC is the first critical step in the interaction between HSC and the microenvironment of the BM. As a consequence, murine models allowing the evaluation of the structural relationship between migrating HSC, the endosteal bone surface, and the vascular components of the BM enhance our understanding of hematopoietic regulation.",

author = "Jochen Grassinger and Susan Nilsson",

year = "2011",

doi = "10.1007/978-1-61779-145-1",

language = "English",

isbn = "978-1-61779-144-4",

pages = "197 -- 214",

editor = "M-D Filippi and H Geiger",

booktitle = "Stem Cell Migration - Methods and Protocols",

publisher = "Humana Press",

address = "United States of America",

edition = "1st",

}

Grassinger, J & Nilsson, S 2011, Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium. in M-D Filippi & H Geiger (eds), Stem Cell Migration - Methods and Protocols. 1st edn, Humana Press, USA, pp. 197 - 214. https://doi.org/10.1007/978-1-61779-145-1

Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium. / Grassinger, Jochen; Nilsson, Susan.

Stem Cell Migration - Methods and Protocols. ed. / M-D Filippi; H Geiger. 1st. ed. USA : Humana Press, 2011. p. 197 - 214.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other

TY - CHAP

T1 - Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium

AU - Grassinger, Jochen

AU - Nilsson, Susan

PY - 2011

Y1 - 2011

N2 - The tracking of immunofluorescent labeled hematopoietic stem and progenitor cells (HSC/HPC) within the bone marrow (BM) cavity allows the assessment of the regulatory processes involved in transendothelial migration, trans-marrow migration, and finally lodgement into the HSC niche. This is of interest as the extracellular and cellular components involved in the regulation of HSC quiescence and differentiation are still not completely understood. Homing of transplanted HSC is the first critical step in the interaction between HSC and the microenvironment of the BM. As a consequence, murine models allowing the evaluation of the structural relationship between migrating HSC, the endosteal bone surface, and the vascular components of the BM enhance our understanding of hematopoietic regulation.

AB - The tracking of immunofluorescent labeled hematopoietic stem and progenitor cells (HSC/HPC) within the bone marrow (BM) cavity allows the assessment of the regulatory processes involved in transendothelial migration, trans-marrow migration, and finally lodgement into the HSC niche. This is of interest as the extracellular and cellular components involved in the regulation of HSC quiescence and differentiation are still not completely understood. Homing of transplanted HSC is the first critical step in the interaction between HSC and the microenvironment of the BM. As a consequence, murine models allowing the evaluation of the structural relationship between migrating HSC, the endosteal bone surface, and the vascular components of the BM enhance our understanding of hematopoietic regulation.

U2 - 10.1007/978-1-61779-145-1

DO - 10.1007/978-1-61779-145-1

M3 - Chapter (Book)

SN - 978-1-61779-144-4

SP - 197

EP - 214

BT - Stem Cell Migration - Methods and Protocols

A2 - Filippi, M-D

A2 - Geiger, H

PB - Humana Press

CY - USA

ER -

Grassinger J, Nilsson S. Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium. In Filippi M-D, Geiger H, editors, Stem Cell Migration - Methods and Protocols. 1st ed. USA: Humana Press. 2011. p. 197 - 214 https://doi.org/10.1007/978-1-61779-145-1