Methods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endothelium

Jochen Grassinger, Susan Nilsson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

13 Citations (Scopus)


The tracking of immunofluorescent labeled hematopoietic stem and progenitor cells (HSC/HPC) within the bone marrow (BM) cavity allows the assessment of the regulatory processes involved in transendothelial migration, trans-marrow migration, and finally lodgement into the HSC niche. This is of interest as the extracellular and cellular components involved in the regulation of HSC quiescence and differentiation are still not completely understood. Homing of transplanted HSC is the first critical step in the interaction between HSC and the microenvironment of the BM. As a consequence, murine models allowing the evaluation of the structural relationship between migrating HSC, the endosteal bone surface, and the vascular components of the BM enhance our understanding of hematopoietic regulation.
Original languageEnglish
Title of host publicationStem Cell Migration - Methods and Protocols
EditorsM-D Filippi, H Geiger
Place of PublicationUSA
PublisherHumana Press
Pages197 - 214
Number of pages18
ISBN (Print)978-1-61779-144-4
Publication statusPublished - 2011

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