Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo

Gavin C. Higgins, Tuong-Vi Nguyen, Georg Ramm, Melinda T. Coughlan

Research output: Contribution to journalReview ArticleOtherpeer-review

1 Citation (Scopus)

Abstract

The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
JournalNephrology
Volume23
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • autophagic flux
  • autophagosomes
  • autophagy
  • ex vivo
  • renal cortex

Equipment

Ramaciotti Centre for Cryo-Electron Microscopy

Georg Ramm (Manager), Viola M.J. Oorschot (Operator), Simon Andrew Crawford (Operator), Hariprasad Venugopal (Operator), Joan Marea Clark (Operator) & Gediminas Gervinskas (Operator)

Office of the Vice-Provost (Research and Research Infrastructure)

Facility/equipment: Facility

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