Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo

Gavin C. Higgins; Tuong-Vi Nguyen; Georg Ramm; Melinda T. Coughlan

doi:10.1111/nep.13251

Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo

Gavin C. Higgins, Tuong-Vi Nguyen, Georg Ramm, Melinda T. Coughlan

Research output: Contribution to journal › Review Article › Other › peer-review

1 Citation (Scopus)

Abstract

The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.

Original language	English
Pages (from-to)	815-820
Number of pages	6
Journal	Nephrology
Volume	23
Issue number	9
DOIs	https://doi.org/10.1111/nep.13251
Publication status	Published - 1 Sept 2018

Keywords

autophagic flux
autophagosomes
autophagy
ex vivo
renal cortex

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10.1111/nep.13251

250853191-oaFinal published version, 1.27 MB

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abstract = "The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.",

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AU - Ramm, Georg

AU - Coughlan, Melinda T.

PY - 2018/9/1

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N2 - The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.

AB - The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.

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KW - autophagosomes

KW - autophagy

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KW - renal cortex

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ER -

Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo

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Keywords

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Ramaciotti Centre for Cryo-Electron Microscopy

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Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo

Abstract

Keywords

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Ramaciotti Centre for Cryo-Electron Microscopy

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