Methods for dynamic synchrotron X-ray respiratory imaging in live animals

Kaye Susannah Morgan, David Parsons, Patricia Cmielewski, Alexandra McCarron, Regine Gradl, Nigel Farrow, Karen Siu, Akihisa Takeuchi, Yoshio Suzuki, Kentaro Uesugi, Masayuki Uesugi, Naoto Yagi, Chris Hall, Mitzi Klein, Anton Maksimenko, Andrew Stevenson, Daniel Hausermann, Martin Dierolf, Franz Pfeifferb, Martin Donnelleyd

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Small-animal physiology studies are typically complicated, but the level of complexity is greatly increased when performing live-animal X-ray imaging studies at synchrotron and compact light sources. This group has extensive experience in these types of studies at the SPring-8 and Australian synchrotrons, as well as the Munich Compact Light Source. These experimental settings produce unique challenges. Experiments are always performed in an isolated radiation enclosure not specifically designed for live-animal imaging. This requires equipment adapted to physiological monitoring and test-substance delivery, as well as shuttering to reduce the radiation dose. Experiment designs must also take into account the fixed location, size and orientation of the X-ray beam. This article describes the techniques developed to overcome the challenges involved in respiratory X-ray imaging of live animals at synchrotrons, now enabling increasingly sophisticated imaging protocols.

Original languageEnglish
Pages (from-to)164-175
Number of pages12
JournalJournal of Synchrotron Radiation
Volume27
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • airways
  • inverse Compton source
  • lungs
  • mouse
  • phase contrast
  • phase-contrast imaging
  • rat
  • trachea

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