Methodology to examine kidney development

Karen M. Moritz, Marelyn Wintour-Coghlan, M. Jane Black, John F. Bertram, Georgina Caruana

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

1 Citation (Scopus)

Abstract

Whole metanephric organ culture, developed by Clifford Grobstein in the 1950s (Grobstein 1956) has been the most important in vitro model system to date for studying kidney development. With this technique, whole rat or mouse metanephroi are cultured in defined media or in the presence of serum from approximately 13.5 or 11.5 dpc, respectively, when the ureteric tree contains just a few branches and the kidney contains no mature nephrons. The metanephroi are cultured at an air-media interface on Transwell filters for up to approximately 6 days, by which time they typically contain at least 50 glomeruli at different stages of maturation, and at least this number of ureteric branches. Developing glomeruli can be visualized and quantified by whole-mount immunostaining the cultures with WT-1, which is a marker of developing podocytes. The development of the ureteric tree can be visualized by immunostaining with calbindin-D28K-or cytokeratin (Fig. 7). Alternatively, metanephroi from Hoxb7/GFP transgenic mice in which the entire ureteric epithelium expresses green fluorescent protein (GFP) can be cultured to monitor the development of the ureteric epithelium (see Sect. 5.5 below).

Original languageEnglish
Title of host publicationFactors Influencing Mammalian Kidney Development
Subtitle of host publicationImplications for Health in Adult Life
Pages18-26
Number of pages9
DOIs
Publication statusPublished - 3 Jun 2008

Publication series

NameAdvances in Anatomy Embryology and Cell Biology
Volume196
ISSN (Print)0301-5556

Cite this

Moritz, K. M., Wintour-Coghlan, M., Black, M. J., Bertram, J. F., & Caruana, G. (2008). Methodology to examine kidney development. In Factors Influencing Mammalian Kidney Development: Implications for Health in Adult Life (pp. 18-26). (Advances in Anatomy Embryology and Cell Biology; Vol. 196). https://doi.org/10.1007/978-3-540-77768-7_5
Moritz, Karen M. ; Wintour-Coghlan, Marelyn ; Black, M. Jane ; Bertram, John F. ; Caruana, Georgina. / Methodology to examine kidney development. Factors Influencing Mammalian Kidney Development: Implications for Health in Adult Life. 2008. pp. 18-26 (Advances in Anatomy Embryology and Cell Biology).
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Moritz, KM, Wintour-Coghlan, M, Black, MJ, Bertram, JF & Caruana, G 2008, Methodology to examine kidney development. in Factors Influencing Mammalian Kidney Development: Implications for Health in Adult Life. Advances in Anatomy Embryology and Cell Biology, vol. 196, pp. 18-26. https://doi.org/10.1007/978-3-540-77768-7_5

Methodology to examine kidney development. / Moritz, Karen M.; Wintour-Coghlan, Marelyn; Black, M. Jane; Bertram, John F.; Caruana, Georgina.

Factors Influencing Mammalian Kidney Development: Implications for Health in Adult Life. 2008. p. 18-26 (Advances in Anatomy Embryology and Cell Biology; Vol. 196).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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Moritz KM, Wintour-Coghlan M, Black MJ, Bertram JF, Caruana G. Methodology to examine kidney development. In Factors Influencing Mammalian Kidney Development: Implications for Health in Adult Life. 2008. p. 18-26. (Advances in Anatomy Embryology and Cell Biology). https://doi.org/10.1007/978-3-540-77768-7_5