TY - JOUR
T1 - Method validation in quantitative analysis of phase I and phase II metabolites of mitragynine in human urine using liquid chromatography-tandem mass spectrometry
AU - Lee, Mei Jin
AU - Ramanathan, Surash
AU - Mansor, Sharif Mahsufi
AU - Yeong, Keng Yoon
AU - Tan, Soo Choon
N1 - Funding Information:
This research project was supported by HiCOE grants ( 311.CIPPM:4401005 and 311/CDADAH/4401009 ) by Ministry of Higher Education (MOHE) , Malaysia. MJL was supported by the Ministry of Education (Malaysia) MYBRAIN15 scholarship.
Funding Information:
This research project was supported by HiCOE grants (311.CIPPM:4401005 and 311/CDADAH/4401009) by Ministry of Higher Education (MOHE), Malaysia. MJL was supported by the Ministry of Education (Malaysia) MYBRAIN15 scholarship.
Publisher Copyright:
© 2017 Elsevier Inc.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/2/15
Y1 - 2018/2/15
N2 - A method using solid phase extraction and liquid chromatography-tandem mass spectrometry to quantitatively detect mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine in human urine samples was developed and validated. The relevant metabolites were identified using multiple reaction monitoring in positive ionization mode using nalorphine as an internal standard. The method was validated for accuracy, precision, recovery, linearity, and lower limit of quantitation. The intra- and inter-day accuracy and precision were found in the range of 83.6–117.5% with coefficient of variation less than 13%. The percentage of recovery for mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine was within the range of 80.1–118.9%. The lower limit of quantification was 1 ng/mL for mitragynine, 2 ng/mL for 16-carboxy mitragynine, and 50 ng/mL for 9-O-demethyl mitragynine. The developed method was reproducible, high precision and accuracy with good linearity and recovery for mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine in human urine.
AB - A method using solid phase extraction and liquid chromatography-tandem mass spectrometry to quantitatively detect mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine in human urine samples was developed and validated. The relevant metabolites were identified using multiple reaction monitoring in positive ionization mode using nalorphine as an internal standard. The method was validated for accuracy, precision, recovery, linearity, and lower limit of quantitation. The intra- and inter-day accuracy and precision were found in the range of 83.6–117.5% with coefficient of variation less than 13%. The percentage of recovery for mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine was within the range of 80.1–118.9%. The lower limit of quantification was 1 ng/mL for mitragynine, 2 ng/mL for 16-carboxy mitragynine, and 50 ng/mL for 9-O-demethyl mitragynine. The developed method was reproducible, high precision and accuracy with good linearity and recovery for mitragynine, 16-carboxy mitragynine, and 9-O-demethyl mitragynine in human urine.
KW - Human urine
KW - Kratom
KW - Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
KW - Metabolites
KW - Solid phase extraction (SPE)
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=85038105842&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2017.12.021
DO - 10.1016/j.ab.2017.12.021
M3 - Article
C2 - 29248503
AN - SCOPUS:85038105842
VL - 543
SP - 146
EP - 161
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
ER -