Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins

Juhsien Nienaber; Batu Kuinkel; Michael Clarke-Pearson; Supaporn Lamlertthon; Lawrence Park; Thomas Rude; Steve Barriere; Christopher Woods; Vivian Chu; Mercedes Marin; Suzana Bukovski; Patricia Garcia; G R Corey; Tony Korman; Thanh Doco-Lecompte; David Murdoch; Barth Reller; Vance Fowler

doi:10.1093/infdis/jir389

Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins

Juhsien Nienaber, Batu Kuinkel, Michael Clarke-Pearson, Supaporn Lamlertthon, Lawrence Park, Thomas Rude, Steve Barriere, Christopher Woods, Vivian Chu, Mercedes Marin, Suzana Bukovski, Patricia Garcia, G R Corey, Tony Korman, Thanh Doco-Lecompte, David Murdoch, Barth Reller, Vance Fowler

Medicine Monash Health

Research output: Contribution to journal › Article › Research › peer-review

104 Citations (Scopus)

Abstract

Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity. METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes. RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5 vs 6.2 ; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P <.0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P

Original language	English
Pages (from-to)	704 - 713
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	204
Issue number	5
DOIs	https://doi.org/10.1093/infdis/jir389
Publication status	Published - 2011

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Nienaber, J., Kuinkel, B., Clarke-Pearson, M., Lamlertthon, S., Park, L., Rude, T., Barriere, S., Woods, C., Chu, V., Marin, M., Bukovski, S., Garcia, P., Corey, G. R., Korman, T., Doco-Lecompte, T., Murdoch, D., Reller, B., & Fowler, V. (2011). Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins. Journal of Infectious Diseases, 204(5), 704 - 713. https://doi.org/10.1093/infdis/jir389

Nienaber, Juhsien ; Kuinkel, Batu ; Clarke-Pearson, Michael ; Lamlertthon, Supaporn ; Park, Lawrence ; Rude, Thomas ; Barriere, Steve ; Woods, Christopher ; Chu, Vivian ; Marin, Mercedes ; Bukovski, Suzana ; Garcia, Patricia ; Corey, G R ; Korman, Tony ; Doco-Lecompte, Thanh ; Murdoch, David ; Reller, Barth ; Fowler, Vance. / Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins. In: Journal of Infectious Diseases. 2011 ; Vol. 204, No. 5. pp. 704 - 713.

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title = "Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins",

abstract = "Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity. METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes. RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5 vs 6.2 ; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P <.0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P",

author = "Juhsien Nienaber and Batu Kuinkel and Michael Clarke-Pearson and Supaporn Lamlertthon and Lawrence Park and Thomas Rude and Steve Barriere and Christopher Woods and Vivian Chu and Mercedes Marin and Suzana Bukovski and Patricia Garcia and Corey, {G R} and Tony Korman and Thanh Doco-Lecompte and David Murdoch and Barth Reller and Vance Fowler",

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Nienaber, J, Kuinkel, B, Clarke-Pearson, M, Lamlertthon, S, Park, L, Rude, T, Barriere, S, Woods, C, Chu, V, Marin, M, Bukovski, S, Garcia, P, Corey, GR, Korman, T, Doco-Lecompte, T, Murdoch, D, Reller, B & Fowler, V 2011, 'Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins', Journal of Infectious Diseases, vol. 204, no. 5, pp. 704 - 713. https://doi.org/10.1093/infdis/jir389

Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins. / Nienaber, Juhsien; Kuinkel, Batu; Clarke-Pearson, Michael; Lamlertthon, Supaporn; Park, Lawrence; Rude, Thomas; Barriere, Steve; Woods, Christopher; Chu, Vivian; Marin, Mercedes; Bukovski, Suzana; Garcia, Patricia; Corey, G R; Korman, Tony; Doco-Lecompte, Thanh; Murdoch, David; Reller, Barth; Fowler, Vance.

In: Journal of Infectious Diseases, Vol. 204, No. 5, 2011, p. 704 - 713.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins

AU - Nienaber, Juhsien

AU - Kuinkel, Batu

AU - Clarke-Pearson, Michael

AU - Lamlertthon, Supaporn

AU - Park, Lawrence

AU - Rude, Thomas

AU - Barriere, Steve

AU - Woods, Christopher

AU - Chu, Vivian

AU - Marin, Mercedes

AU - Bukovski, Suzana

AU - Garcia, Patricia

AU - Corey, G R

AU - Korman, Tony

AU - Doco-Lecompte, Thanh

AU - Murdoch, David

AU - Reller, Barth

AU - Fowler, Vance

PY - 2011

Y1 - 2011

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JF - Journal of Infectious Diseases

SN - 0022-1899

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