Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins

Juhsien Nienaber, Batu Kuinkel, Michael Clarke-Pearson, Supaporn Lamlertthon, Lawrence Park, Thomas Rude, Steve Barriere, Christopher Woods, Vivian Chu, Mercedes Marin, Suzana Bukovski, Patricia Garcia, G R Corey, Tony Korman, Thanh Doco-Lecompte, David Murdoch, Barth Reller, Vance Fowler

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Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity. METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes. RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5 vs 6.2 ; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P <.0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P
Original languageEnglish
Pages (from-to)704 - 713
Number of pages10
JournalThe Journal of Infectious Diseases
Issue number5
Publication statusPublished - 2011

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