Metal complexes as tools for structural biology

Michael David Lee, Bimbil Graham, James David Swarbrick

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

Structural biologists currently have at their disposal a diverse and powerful range of tools and techniques that can be drawn upon to generate structural models. X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy provide high-resolution structures of proteins and nucleic acids with atomic level of detail. Within X-ray crystallographic studies, metal complexes have been employed for heavy-atom derivatization of proteins, enabling extraction of phase information, whilst NMR spectroscopic analysis of proteins and other macromolecules has benefited greatly from the introduction of so-called "lanthanide-binding tags". Selected luminescent lanthanide complexes have found application as "donors" in fluorescence resonance energy transfer (FRET) spectroscopic measurements, greatly extending the upper limit and accuracy of the distances that can be measured. A modification of conventional FRET that allows the measurement of longer distances with greater accuracy is luminescence resonance energy transfer (LRET).

Original languageEnglish
Title of host publicationInorganic chemical biology: Principles, techniques and applications
EditorsGilles Gasser
Place of PublicationChichester UK
PublisherJohn Wiley & Sons
Pages37-62
Number of pages26
ISBN (Electronic)9781118682975
ISBN (Print)9781118510025
DOIs
Publication statusPublished - 2014

Cite this

Lee, M. D., Graham, B., & Swarbrick, J. D. (2014). Metal complexes as tools for structural biology. In G. Gasser (Ed.), Inorganic chemical biology: Principles, techniques and applications (pp. 37-62). Chichester UK: John Wiley & Sons. https://doi.org/10.1002/9781118682975, https://doi.org/10.1002/9781118682975.ch2
Lee, Michael David ; Graham, Bimbil ; Swarbrick, James David. / Metal complexes as tools for structural biology. Inorganic chemical biology: Principles, techniques and applications. editor / Gilles Gasser. Chichester UK : John Wiley & Sons, 2014. pp. 37-62
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Lee, MD, Graham, B & Swarbrick, JD 2014, Metal complexes as tools for structural biology. in G Gasser (ed.), Inorganic chemical biology: Principles, techniques and applications. John Wiley & Sons, Chichester UK, pp. 37-62. https://doi.org/10.1002/9781118682975, https://doi.org/10.1002/9781118682975.ch2

Metal complexes as tools for structural biology. / Lee, Michael David; Graham, Bimbil; Swarbrick, James David.

Inorganic chemical biology: Principles, techniques and applications. ed. / Gilles Gasser. Chichester UK : John Wiley & Sons, 2014. p. 37-62.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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AB - Structural biologists currently have at their disposal a diverse and powerful range of tools and techniques that can be drawn upon to generate structural models. X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy provide high-resolution structures of proteins and nucleic acids with atomic level of detail. Within X-ray crystallographic studies, metal complexes have been employed for heavy-atom derivatization of proteins, enabling extraction of phase information, whilst NMR spectroscopic analysis of proteins and other macromolecules has benefited greatly from the introduction of so-called "lanthanide-binding tags". Selected luminescent lanthanide complexes have found application as "donors" in fluorescence resonance energy transfer (FRET) spectroscopic measurements, greatly extending the upper limit and accuracy of the distances that can be measured. A modification of conventional FRET that allows the measurement of longer distances with greater accuracy is luminescence resonance energy transfer (LRET).

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Lee MD, Graham B, Swarbrick JD. Metal complexes as tools for structural biology. In Gasser G, editor, Inorganic chemical biology: Principles, techniques and applications. Chichester UK: John Wiley & Sons. 2014. p. 37-62 https://doi.org/10.1002/9781118682975, https://doi.org/10.1002/9781118682975.ch2