Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) in relation to estrogenic response

C. Lindholst, P. M. Wynne, P. Marriott, S. N. Pedersen, P. Bjerregaard

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65 Citations (Scopus)

Abstract

The kinetics of bisphenol A (BPA) were investigated in zebrafish (Danio rerio) exposed to 100 μg BPA/l. BPA uptake was measured during a 7-day period followed by an elimination phase of similar duration. After 2, 6, 12, 24, 48, 72, 120 and 168 h of uptake/elimination, fish were analysed for their content of BPA, bisphenol A glucuronic acid (BPAGA) and bisphenol A sulfate (BPAS). Within the first 24 h steady state levels of BPA, BPAGA and BPAS were reached and the total body concentrations were calculated to be 569, 12600 and 39.9 ng/g fish, respectively. Elimination rates of the three compounds in zebrafish were estimated by fitting the data to a compartment model. An initial rapid elimination phase was observed for BPA and BPAS with total body half lives (T1/2) of <1.1 h and 30 min, followed by a slower second elimination phase with T1/2 values of 139 and 71 h, respectively. Excretion of BPAGA occurred from a single compartment with a T1/2 of 35 h. The steady state concentration of BPA and its metabolites were investigated in rainbow trout (Oncorhynchus mykiss) exposed to 100 μg BPA/l. The toxicokinetic parameters from zebrafish and rainbow trout were compared; including previously published data on the rainbow trout. The data indicate that the smaller estrogenic sensitivity observed for the zebrafish may be caused by a more rapid metabolism of BPA in the zebrafish liver.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume135
Issue number2
DOIs
Publication statusPublished - 1 Jun 2003
Externally publishedYes

Keywords

  • Bisphenol A
  • Bisphenol A glucuronic acid
  • Bisphenol A sulfate
  • Endocrine disrupters
  • LC-MS
  • Metabolism
  • Rainbow trout
  • Zebrafish

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