TY - JOUR
T1 - Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) in relation to estrogenic response
AU - Lindholst, C.
AU - Wynne, P. M.
AU - Marriott, P.
AU - Pedersen, S. N.
AU - Bjerregaard, P.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - The kinetics of bisphenol A (BPA) were investigated in zebrafish (Danio rerio) exposed to 100 μg BPA/l. BPA uptake was measured during a 7-day period followed by an elimination phase of similar duration. After 2, 6, 12, 24, 48, 72, 120 and 168 h of uptake/elimination, fish were analysed for their content of BPA, bisphenol A glucuronic acid (BPAGA) and bisphenol A sulfate (BPAS). Within the first 24 h steady state levels of BPA, BPAGA and BPAS were reached and the total body concentrations were calculated to be 569, 12600 and 39.9 ng/g fish, respectively. Elimination rates of the three compounds in zebrafish were estimated by fitting the data to a compartment model. An initial rapid elimination phase was observed for BPA and BPAS with total body half lives (T1/2) of <1.1 h and 30 min, followed by a slower second elimination phase with T1/2 values of 139 and 71 h, respectively. Excretion of BPAGA occurred from a single compartment with a T1/2 of 35 h. The steady state concentration of BPA and its metabolites were investigated in rainbow trout (Oncorhynchus mykiss) exposed to 100 μg BPA/l. The toxicokinetic parameters from zebrafish and rainbow trout were compared; including previously published data on the rainbow trout. The data indicate that the smaller estrogenic sensitivity observed for the zebrafish may be caused by a more rapid metabolism of BPA in the zebrafish liver.
AB - The kinetics of bisphenol A (BPA) were investigated in zebrafish (Danio rerio) exposed to 100 μg BPA/l. BPA uptake was measured during a 7-day period followed by an elimination phase of similar duration. After 2, 6, 12, 24, 48, 72, 120 and 168 h of uptake/elimination, fish were analysed for their content of BPA, bisphenol A glucuronic acid (BPAGA) and bisphenol A sulfate (BPAS). Within the first 24 h steady state levels of BPA, BPAGA and BPAS were reached and the total body concentrations were calculated to be 569, 12600 and 39.9 ng/g fish, respectively. Elimination rates of the three compounds in zebrafish were estimated by fitting the data to a compartment model. An initial rapid elimination phase was observed for BPA and BPAS with total body half lives (T1/2) of <1.1 h and 30 min, followed by a slower second elimination phase with T1/2 values of 139 and 71 h, respectively. Excretion of BPAGA occurred from a single compartment with a T1/2 of 35 h. The steady state concentration of BPA and its metabolites were investigated in rainbow trout (Oncorhynchus mykiss) exposed to 100 μg BPA/l. The toxicokinetic parameters from zebrafish and rainbow trout were compared; including previously published data on the rainbow trout. The data indicate that the smaller estrogenic sensitivity observed for the zebrafish may be caused by a more rapid metabolism of BPA in the zebrafish liver.
KW - Bisphenol A
KW - Bisphenol A glucuronic acid
KW - Bisphenol A sulfate
KW - Endocrine disrupters
KW - LC-MS
KW - Metabolism
KW - Rainbow trout
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=0038160736&partnerID=8YFLogxK
U2 - 10.1016/S1532-0456(03)00088-7
DO - 10.1016/S1532-0456(03)00088-7
M3 - Article
C2 - 12860056
AN - SCOPUS:0038160736
SN - 1532-0456
VL - 135
SP - 169
EP - 177
JO - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
JF - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
IS - 2
ER -