Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

Wei Jiang, David Hernández Villamor, Huadong Peng, Jian Chen, Long Liu, Victoria Haritos, Rodrigo Ledesma-Amaro

Research output: Contribution to journalReview ArticleResearchpeer-review

35 Citations (Scopus)


One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing industry and have recently gained attention owing to their natural abundance, low production cost and availability as industrial by-products. However, native pathways to utilize these substrates are absent in most biotechnologically relevant microorganisms. Recent advances in synthetic biology, genome engineering and laboratory evolution are enabling the first steps towards the creation of synthetic C1-utilizing microorganisms. Here, we briefly review the native metabolism of methane, methanol, CO2, CO and formate, and how these C1-utilizing pathways can be engineered into heterologous hosts. In addition, this review analyses the potential, the challenges and the perspectives of C1-based biomanufacturing. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)845-855
Number of pages11
JournalNature Chemical Biology
Issue number8
Publication statusPublished - 26 Jul 2021

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