TY - JOUR
T1 - Mesenchymal stem/stromal cells in post-menopausal endometrium
AU - Ulrich, Daniela
AU - Tan, Ker Sin
AU - Deane, James Antony
AU - Schwab, Kjiana Elkje
AU - Cheong, Amy
AU - Rosamilia, Anna
AU - Gargett, Caroline Eve
PY - 2014
Y1 - 2014
N2 - STUDY QUESTION:
Does post-menopausal endometrium contain mesenchymal stem/stromal cells (MSC) that have adult stem cell properties and can be prospectively isolated from a biopsy?
SUMMARY ANSWER:
Perivascular W5C5(+) cells isolated from post-menopausal endometrial biopsies displayed characteristic MSC properties of clonogenicity, multipotency and surface phenotype irrespective of whether the women were or were not pre-treated with estrogen to regenerate the endometrium.
WHAT IS KNOWN ALREADY:
Recently MSCs have been identified in human premenopausal endometrium, and can be prospectively isolated using a single marker, W5C5/SUSD2.
STUDY DESIGN, SIZE, DURATION:
Endometrial tissue of both the functional and basal layers, from 17 premenopausal (pre-MP) women, 19 post-menopausal (post-MP) women without hormonal treatment and 15 post-menopausal women on estrogen replacement therapy (post-MP+ E2), was collected through a prospective phase IV clinical trial over 2 years.
PARTICIPANTS/MATERIALS, SETTING, METHODS:
Post-menopausal women
AB - STUDY QUESTION:
Does post-menopausal endometrium contain mesenchymal stem/stromal cells (MSC) that have adult stem cell properties and can be prospectively isolated from a biopsy?
SUMMARY ANSWER:
Perivascular W5C5(+) cells isolated from post-menopausal endometrial biopsies displayed characteristic MSC properties of clonogenicity, multipotency and surface phenotype irrespective of whether the women were or were not pre-treated with estrogen to regenerate the endometrium.
WHAT IS KNOWN ALREADY:
Recently MSCs have been identified in human premenopausal endometrium, and can be prospectively isolated using a single marker, W5C5/SUSD2.
STUDY DESIGN, SIZE, DURATION:
Endometrial tissue of both the functional and basal layers, from 17 premenopausal (pre-MP) women, 19 post-menopausal (post-MP) women without hormonal treatment and 15 post-menopausal women on estrogen replacement therapy (post-MP+ E2), was collected through a prospective phase IV clinical trial over 2 years.
PARTICIPANTS/MATERIALS, SETTING, METHODS:
Post-menopausal women
UR - http://humrep.oxfordjournals.org/content/29/9/1895.full.pdf+html
U2 - 10.1093/humrep/deu159
DO - 10.1093/humrep/deu159
M3 - Article
SN - 0268-1161
VL - 29
SP - 1895
EP - 1905
JO - Human Reproduction
JF - Human Reproduction
IS - 9
ER -