Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren R. Clements, Peng Yuan Wang, Frances Harding, Wei Bor Tsai, Helmut Thissen, Nicolas H. Voelcker

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)


Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface. 

Original languageEnglish
Pages (from-to)1440-1445
Number of pages6
JournalPhysica Status Solidi A: Applications and Materials Science
Issue number6
Publication statusPublished - Jun 2011
Externally publishedYes


  • biomaterial
  • electrochemistry
  • gradient surfaces
  • porous silicon
  • surface chemistry

Cite this