Abstract
Repair after damage is essential for tissue homeostasis. Postmenstrual endometrial repair is a cyclical manifestation of rapid, scar-free, tissue repair taking ∼3-5 d. Skin repair after wounding is slower (∼2 wk). In the case of chronic wounds, it takes months to years to restore integrity. Herein, the unique "rapid-repair" endometrial environment is translated to the "slower repair" skin environment. Menstrual fluid (MF), the milieu of postmenstrual endometrial repair, facilitates healing of endometrial and keratinocyte "wounds" in vitro, promoting cellular adhesion and migration, stimulates keratinocyte migration in an ex vivo human skin reconstruct model, and promotes re-epithelialization in an in vivo porcine wound model. Proteomic analysis of MF identified a large number of proteins: migration inhibitory factor, neutrophil gelatinase-associated lipocalin, follistatin like-1, chemokine ligand-20, and secretory leukocyte protease inhibitor were selected for further investigation. Functionally, they promote repair of endometrial and keratinocyte wounds by promoting migration. Translation of these and other MF factors into a migration-inducing treatment paradigm could provide novel treatments for tissue repair.
| Original language | English |
|---|---|
| Pages (from-to) | 584-605 |
| Number of pages | 22 |
| Journal | FASEB Journal |
| Volume | 33 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Keywords
- Menstruation
- Migration
- Proteomics
Cite this
}
Menstrual fluid factors facilitate tissue repair : identification and functional action in endometrial and skin repair. / Evans, Jemma; Infusini, Giuseppe; McGovern, Jacqui; Cuttle, Leila; Webb, Andrew; Nebl, Thomas; Milla, Liz; Kimble, Roy; Kempf, Margit; Andrews, Christine J.; Leavesley, David; Salamonsen, Lois A.
In: FASEB Journal, Vol. 33, No. 1, 01.01.2019, p. 584-605.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Menstrual fluid factors facilitate tissue repair
T2 - identification and functional action in endometrial and skin repair
AU - Evans, Jemma
AU - Infusini, Giuseppe
AU - McGovern, Jacqui
AU - Cuttle, Leila
AU - Webb, Andrew
AU - Nebl, Thomas
AU - Milla, Liz
AU - Kimble, Roy
AU - Kempf, Margit
AU - Andrews, Christine J.
AU - Leavesley, David
AU - Salamonsen, Lois A.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Repair after damage is essential for tissue homeostasis. Postmenstrual endometrial repair is a cyclical manifestation of rapid, scar-free, tissue repair taking ∼3-5 d. Skin repair after wounding is slower (∼2 wk). In the case of chronic wounds, it takes months to years to restore integrity. Herein, the unique "rapid-repair" endometrial environment is translated to the "slower repair" skin environment. Menstrual fluid (MF), the milieu of postmenstrual endometrial repair, facilitates healing of endometrial and keratinocyte "wounds" in vitro, promoting cellular adhesion and migration, stimulates keratinocyte migration in an ex vivo human skin reconstruct model, and promotes re-epithelialization in an in vivo porcine wound model. Proteomic analysis of MF identified a large number of proteins: migration inhibitory factor, neutrophil gelatinase-associated lipocalin, follistatin like-1, chemokine ligand-20, and secretory leukocyte protease inhibitor were selected for further investigation. Functionally, they promote repair of endometrial and keratinocyte wounds by promoting migration. Translation of these and other MF factors into a migration-inducing treatment paradigm could provide novel treatments for tissue repair.
AB - Repair after damage is essential for tissue homeostasis. Postmenstrual endometrial repair is a cyclical manifestation of rapid, scar-free, tissue repair taking ∼3-5 d. Skin repair after wounding is slower (∼2 wk). In the case of chronic wounds, it takes months to years to restore integrity. Herein, the unique "rapid-repair" endometrial environment is translated to the "slower repair" skin environment. Menstrual fluid (MF), the milieu of postmenstrual endometrial repair, facilitates healing of endometrial and keratinocyte "wounds" in vitro, promoting cellular adhesion and migration, stimulates keratinocyte migration in an ex vivo human skin reconstruct model, and promotes re-epithelialization in an in vivo porcine wound model. Proteomic analysis of MF identified a large number of proteins: migration inhibitory factor, neutrophil gelatinase-associated lipocalin, follistatin like-1, chemokine ligand-20, and secretory leukocyte protease inhibitor were selected for further investigation. Functionally, they promote repair of endometrial and keratinocyte wounds by promoting migration. Translation of these and other MF factors into a migration-inducing treatment paradigm could provide novel treatments for tissue repair.
KW - Menstruation
KW - Migration
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85059242693&partnerID=8YFLogxK
U2 - 10.1096/fj.201800086R
DO - 10.1096/fj.201800086R
M3 - Article
VL - 33
SP - 584
EP - 605
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 1
ER -