Memory regulatory T cells home to the lung and control influenza A virus infection

Chunni Lu, Damien Zanker, Peter Lock, Xiangrui Jiang, Jieru Deng, Mubing Duan, Chuanxin Liu, Pierre Faou, Michael J. Hickey, Weisan Chen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Memory regulatory T cells (mTregs) have been demonstrated to persist long-term in hosts after the resolution of primary influenza A virus (IAV) infection. However, whether such IAV infection-experienced (IAV-experienced) mTregs differentiate into a phenotypically and functionally distinct Treg subset and what function they play at the infection site remains poorly defined. In this study, we characterized the phenotype, examined the responsiveness and assessed the suppressive function of IAV-experienced memory Tregs (mTregs). In comparison with inexperienced naïve Tregs (nTregs), mTregs exhibited elevated expression of CD39, CD69, CD103, cytotoxic T lymphocyte-associated antigen-4, leukocyte function-associated antigen-1 and programmed cell death-1 and could be activated in an antigen-specific manner in vitro and in vivo. When mTregs and nTregs were adoptively cotransferred into recipient mice, mTregs had a competitive advantage in migrating to the IAV-infected lungs. mTregs were more capable of controlling in vitro proliferation of CD4+ and CD8+ T cells and suppressed CD40 and CD86 upregulation on bone marrow-derived dendritic cells. Adoptively transferred mTregs, but not adoptively transferred nTregs, significantly attenuated body weight loss, lung pathology and immune cell infiltration into the infected lungs after IAV infection. These results suggest that mTregs generated after IAV infection differentiate into a phenotypically distinct and functionally enhanced Treg subset that can be activated in an antigen-specific manner to exert immunosuppression. We propose vaccination to induce such mTregs as a potential novel strategy to protect against severe IAV infection.

Original languageEnglish
Number of pages13
JournalImmunology and Cell Biology
DOIs
Publication statusAccepted/In press - 22 May 2019

Keywords

  • C57BL/6
  • infection-experienced
  • influenza A virus
  • memory regulatory T cells

Cite this

Lu, C., Zanker, D., Lock, P., Jiang, X., Deng, J., Duan, M., ... Chen, W. (Accepted/In press). Memory regulatory T cells home to the lung and control influenza A virus infection. Immunology and Cell Biology. https://doi.org/10.1111/imcb.12271
Lu, Chunni ; Zanker, Damien ; Lock, Peter ; Jiang, Xiangrui ; Deng, Jieru ; Duan, Mubing ; Liu, Chuanxin ; Faou, Pierre ; Hickey, Michael J. ; Chen, Weisan. / Memory regulatory T cells home to the lung and control influenza A virus infection. In: Immunology and Cell Biology. 2019.
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Memory regulatory T cells home to the lung and control influenza A virus infection. / Lu, Chunni; Zanker, Damien; Lock, Peter; Jiang, Xiangrui; Deng, Jieru; Duan, Mubing; Liu, Chuanxin; Faou, Pierre; Hickey, Michael J.; Chen, Weisan.

In: Immunology and Cell Biology, 22.05.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lu, Chunni

AU - Zanker, Damien

AU - Lock, Peter

AU - Jiang, Xiangrui

AU - Deng, Jieru

AU - Duan, Mubing

AU - Liu, Chuanxin

AU - Faou, Pierre

AU - Hickey, Michael J.

AU - Chen, Weisan

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