Membrane proteins associated with sperm-oocyte interaction: A proteomic comparison between Kedah Kelantan (Bos indicus) and Mafriwal (Bos taurus × Bos indicus) Sperm

Ali Ashrafzadeh, Sheila Nathan, Iekhsan Othman, Tee Ting Yee, Saiful Anuar Karsani

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review


Production performance of European cattle breeds has significantly improved through various breeding programs. However, European breeds are more susceptible to heat stress compared to zebu cattle (Bos indicus) as their conception rate can range between 20 to 30% in hot seasons compared to winter. To identify cattle sperm proteins associated with zebu cattle higher fertility and heat tolerance in tropical environments, we utilised a proteomics-based approach to compare sperm from the highly fertile Malaysian indigenous breed, Kedah Kelantan (Bos indicus), with sperm from the sub-fertile crossbreed, Mafriwal (Bos taurus × Bos indicus). Frozen semen of three high performance bulls from each breed was processed to obtain live and pure sperm. Proteins were separated and gel bands were processed by in-gel tryptic digestion. For each breed, mass spectrometry data was acquired over 11 replicates. The analyzed data identified peptides with different expression levels (99% confidence level) and protein identification was determined by targeted MS/MS. Among the identified proteins associated with sperm-oocyte interaction, two proteins were up-regulated in Kedah Kelantan sperm and 7 proteins were up-regulated in or specific to Mafriwal. Our results suggest that the higher fertility of zebu cattle in tropical areas may not be related to more efficient sperm-oocyte interaction. Further analysis of the other regulated proteins in these two breeds may contribute further knowledge on the physiological reason/s for higher fertility and heat tolerance of Zebu cattle in tropical areas.

Original languageEnglish
Title of host publication2013 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2013 Postgraduate Colloquium
Number of pages4
Publication statusPublished - 2013
EventUniversiti Kebangsaan Malaysia Faculty of Science and Technology Post-Graduate Colloquium 2013 - Selangor, Malaysia
Duration: 3 Jul 20134 Jul 2013


ConferenceUniversiti Kebangsaan Malaysia Faculty of Science and Technology Post-Graduate Colloquium 2013


  • Fertility
  • Membrane proteins
  • Sperm
  • Sperm-Oocyte

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