Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood

Michael R. Tallack, Andrew C. Perkins

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

Commitment towards megakaryocyte versus erythroid blood cell lineages occurs in the megakaryocyte-erythroid progenitor, where mutually exclusive expression of either EKLF (Klf1) or Fli1 defines alternative outcomes. Here we show there is a marked increase in the number of circulating platelets in mice lacking the erythroid transcription factor EKLF. In addition, committed erythroid cells retain key signatures of megakaryocytes both on the cell surface and at the mRNA level. We also show that the effect of EKLF on megakaryocyte-erythroid progenitor lineage decision and commitment is cell autonomous in bone marrow reconstitution assays where stem cells lacking EKLF favor the megakaryocyte differentiation pathway. We conclude the megakaryocyte program is aberrantly activated in EKLF null erythroid cells.

Original languageEnglish
Pages (from-to)144-147
Number of pages4
JournalHaematologica
Volume95
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Keywords

  • EKLF
  • Megakaryocyte
  • Megakaryocyte differentiation

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