Mediator subunit 12 is required for neutrophil development in zebrafish

Maria-Cristina Keightley, Judith Layton, John Hayman, Joan Heath, Graham Lieschke

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17 Citations (Scopus)

Abstract

Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
Original languageEnglish
Article numbere23845
Number of pages8
JournalPLoS ONE
Volume6
Issue number8
DOIs
Publication statusPublished - 2011

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