MeCP2 interacts with chromosomal microRNAs in brain

Abdul Waheed Khan, Mark Ziemann, Haloom Rafehi, Scott Maxwell, Giuseppe D. Ciccotosto, Assam El-Osta

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Although methyl CpG binding domain protein-2 (MeCP2) is commonly understood to function as a silencing factor at methylated DNA sequences, recent studies also show that MeCP2 can bind unmethylated sequences and coordinate gene activation. MeCP2 displays broad binding patterns throughout the genome, with high expression levels similar to histone H1 in neurons. Despite its significant presence in the brain, only subtle gene expression changes occur in the absence of MeCP2. This may reflect a more complex regulatory mechanism of MeCP2 to complement chromatin binding. Using an RNA immunoprecipitation of native chromatin technique, we identify MeCP2 interacting microRNAs in mouse primary cortical neurons. In addition, comparison with mRNA sequencing data from Mecp2-null mice suggests that differentially expressed genes may indeed be targeted by MeCP2-interacting microRNAs. These findings highlight the MeCP2 interaction with microRNAs that may modulate its binding with chromatin and regulate gene expression.

Original languageEnglish
Pages (from-to)1028-1037
Number of pages10
JournalEpigenetics
Volume12
Issue number12
DOIs
Publication statusPublished - 2 Dec 2017

Keywords

  • MeCP2
  • microRNAs
  • RNA-chromatin immunoprecipitation

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