TY - JOUR
T1 - Mechanistic insights into class B radical-S-adenosylmethionine methylases
T2 - ubiquitous tailoring enzymes in natural product biosynthesis
AU - Zhou, Shanshan
AU - Alkhalaf, Lona M.
AU - de los Santos, Emmanuel LC
AU - Challis, Gregory L.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Class B radical S-adenosylmethionine (SAM) methylases are notable for their ability to catalyse methylation reactions in the biosynthesis of a wide variety of natural products, including polyketides, ribosomally biosynthesised and post-translationally modified peptides (RiPPs), nonribosomal peptides (NRPs), aminoglycosides, β-lactams, phosphonates, enediynes, aminocoumarins and terpenes. Here, we discuss the diversity of substrates and catalytic mechanism utilised by such enzymes, highlighting the stereochemical course of methylation reactions at un-activated carbon centres and the ability of some members of the family to catalyse multiple methylations.
AB - Class B radical S-adenosylmethionine (SAM) methylases are notable for their ability to catalyse methylation reactions in the biosynthesis of a wide variety of natural products, including polyketides, ribosomally biosynthesised and post-translationally modified peptides (RiPPs), nonribosomal peptides (NRPs), aminoglycosides, β-lactams, phosphonates, enediynes, aminocoumarins and terpenes. Here, we discuss the diversity of substrates and catalytic mechanism utilised by such enzymes, highlighting the stereochemical course of methylation reactions at un-activated carbon centres and the ability of some members of the family to catalyse multiple methylations.
UR - http://www.scopus.com/inward/record.url?scp=84987606159&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2016.08.021
DO - 10.1016/j.cbpa.2016.08.021
M3 - Review Article
C2 - 27632683
AN - SCOPUS:84987606159
SN - 1367-5931
VL - 35
SP - 73
EP - 79
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
ER -