Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16)

Margaret L. Hibbs, Periasamy Selvaraj, Olli Carpén, Timothy A. Springer, Helmut Kuster, Marie Hélène E. Jouvin, Jean Pierre Kinet

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119 Citations (Scopus)

Abstract

Granulocyte and natural killer (NK) cell Fc receptors for immunoglobulin G (CD16) differ in only a few amino acids, yet have phosphatidylinositol glycan (PIG) or polypeptide membrane anchors, respectively. Mutagenesis shows that anchoring is regulated by a serine residue near the PIG anchor attachment site in the extracellular domain. The NK cell isoform was not expressed on the surface of COS cells unless cotransfected with a subunit that was expressed in NK cells and that was identical to the γ subunit of the high affinity IgE Fc receptor (Fc∈RI). However, the CD16 sequence and not expression of the γ subunit is dominant in regulating PIG reanchoring.

Original languageEnglish
Pages (from-to)1608-1611
Number of pages4
JournalScience
Volume246
Issue number4937
DOIs
Publication statusPublished - 1 Jan 1989

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