Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16)

Margaret L. Hibbs, Periasamy Selvaraj, Olli Carpén, Timothy A. Springer, Helmut Kuster, Marie Hélène E. Jouvin, Jean Pierre Kinet

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115 Citations (Scopus)

Abstract

Granulocyte and natural killer (NK) cell Fc receptors for immunoglobulin G (CD16) differ in only a few amino acids, yet have phosphatidylinositol glycan (PIG) or polypeptide membrane anchors, respectively. Mutagenesis shows that anchoring is regulated by a serine residue near the PIG anchor attachment site in the extracellular domain. The NK cell isoform was not expressed on the surface of COS cells unless cotransfected with a subunit that was expressed in NK cells and that was identical to the γ subunit of the high affinity IgE Fc receptor (Fc∈RI). However, the CD16 sequence and not expression of the γ subunit is dominant in regulating PIG reanchoring.

Original languageEnglish
Pages (from-to)1608-1611
Number of pages4
JournalScience
Volume246
Issue number4937
DOIs
Publication statusPublished - 1 Jan 1989

Cite this

Hibbs, M. L., Selvaraj, P., Carpén, O., Springer, T. A., Kuster, H., Jouvin, M. H. E., & Kinet, J. P. (1989). Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16). Science, 246(4937), 1608-1611. https://doi.org/10.1126/science.2531918
Hibbs, Margaret L. ; Selvaraj, Periasamy ; Carpén, Olli ; Springer, Timothy A. ; Kuster, Helmut ; Jouvin, Marie Hélène E. ; Kinet, Jean Pierre. / Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16). In: Science. 1989 ; Vol. 246, No. 4937. pp. 1608-1611.
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abstract = "Granulocyte and natural killer (NK) cell Fc receptors for immunoglobulin G (CD16) differ in only a few amino acids, yet have phosphatidylinositol glycan (PIG) or polypeptide membrane anchors, respectively. Mutagenesis shows that anchoring is regulated by a serine residue near the PIG anchor attachment site in the extracellular domain. The NK cell isoform was not expressed on the surface of COS cells unless cotransfected with a subunit that was expressed in NK cells and that was identical to the γ subunit of the high affinity IgE Fc receptor (Fc∈RI). However, the CD16 sequence and not expression of the γ subunit is dominant in regulating PIG reanchoring.",
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Hibbs, ML, Selvaraj, P, Carpén, O, Springer, TA, Kuster, H, Jouvin, MHE & Kinet, JP 1989, 'Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16)', Science, vol. 246, no. 4937, pp. 1608-1611. https://doi.org/10.1126/science.2531918

Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16). / Hibbs, Margaret L.; Selvaraj, Periasamy; Carpén, Olli; Springer, Timothy A.; Kuster, Helmut; Jouvin, Marie Hélène E.; Kinet, Jean Pierre.

In: Science, Vol. 246, No. 4937, 01.01.1989, p. 1608-1611.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Jouvin, Marie Hélène E.

AU - Kinet, Jean Pierre

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Hibbs ML, Selvaraj P, Carpén O, Springer TA, Kuster H, Jouvin MHE et al. Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16). Science. 1989 Jan 1;246(4937):1608-1611. https://doi.org/10.1126/science.2531918