Mechanism of Shiga Toxin Clustering on Membranes

Weria Pezeshkian, Haifei Gao, Senthil Arumugam, Ulrike Becken, Patricia Bassereau, Jean Claude Florent, John Hjort Ipsen, Ludger Johannes, Julian C. Shillcock

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

The bacterial Shiga toxin interacts with its cellular receptor, the glycosphingolipid globotriaosylceramide (Gb3 or CD77), as a first step to entering target cells. Previous studies have shown that toxin molecules cluster on the plasma membrane, despite the apparent lack of direct interactions between them. The precise mechanism by which this clustering occurs remains poorly defined. Here, we used vesicle and cell systems and computer simulations to show that line tension due to curvature, height, or compositional mismatch, and lipid or solvent depletion cannot drive the clustering of Shiga toxin molecules. By contrast, in coarse-grained computer simulations, a correlation was found between clustering and toxin nanoparticle-driven suppression of membrane fluctuations, and experimentally we observed that clustering required the toxin molecules to be tightly bound to the membrane surface. The most likely interpretation of these findings is that a membrane fluctuation-induced force generates an effective attraction between toxin molecules. Such force would be of similar strength to the electrostatic force at separations around 1 nm, remain strong at distances up to the size of toxin molecules (several nanometers), and persist even beyond. This force is predicted to operate between manufactured nanoparticles providing they are sufficiently rigid and tightly bound to the plasma membrane, thereby suggesting a route for the targeting of nanoparticles to cells for biomedical applications.

Original languageEnglish
Pages (from-to)314-324
Number of pages11
JournalACS Nano
Volume11
Issue number1
DOIs
Publication statusPublished - 24 Jan 2017
Externally publishedYes

Keywords

  • Casimir force
  • clustering
  • endocytosis
  • fluctuation-induced force
  • glycosphingolipid
  • invagination
  • lectin
  • membrane

Cite this

Pezeshkian, W., Gao, H., Arumugam, S., Becken, U., Bassereau, P., Florent, J. C., ... Shillcock, J. C. (2017). Mechanism of Shiga Toxin Clustering on Membranes. ACS Nano, 11(1), 314-324. https://doi.org/10.1021/acsnano.6b05706
Pezeshkian, Weria ; Gao, Haifei ; Arumugam, Senthil ; Becken, Ulrike ; Bassereau, Patricia ; Florent, Jean Claude ; Ipsen, John Hjort ; Johannes, Ludger ; Shillcock, Julian C. / Mechanism of Shiga Toxin Clustering on Membranes. In: ACS Nano. 2017 ; Vol. 11, No. 1. pp. 314-324.
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Pezeshkian, W, Gao, H, Arumugam, S, Becken, U, Bassereau, P, Florent, JC, Ipsen, JH, Johannes, L & Shillcock, JC 2017, 'Mechanism of Shiga Toxin Clustering on Membranes', ACS Nano, vol. 11, no. 1, pp. 314-324. https://doi.org/10.1021/acsnano.6b05706

Mechanism of Shiga Toxin Clustering on Membranes. / Pezeshkian, Weria; Gao, Haifei; Arumugam, Senthil; Becken, Ulrike; Bassereau, Patricia; Florent, Jean Claude; Ipsen, John Hjort; Johannes, Ludger; Shillcock, Julian C.

In: ACS Nano, Vol. 11, No. 1, 24.01.2017, p. 314-324.

Research output: Contribution to journalArticleResearchpeer-review

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Pezeshkian W, Gao H, Arumugam S, Becken U, Bassereau P, Florent JC et al. Mechanism of Shiga Toxin Clustering on Membranes. ACS Nano. 2017 Jan 24;11(1):314-324. https://doi.org/10.1021/acsnano.6b05706