Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Aurelien Forget, Andreas Blaeser, Florian Miessmer, Marius Köpf, Daniela F.Duarte Campos, Nicolas H. Voelcker, Anton Blencowe, Horst Fischer, V. Prasad Shastri

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35 Citations (Scopus)

Abstract

This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5-230 Pa) while ensuring almost no change to the shear viscosity (10-17 mPa) of the bioink solution; thus enabling the fabrication of 3D structures made of different mechanical domains under identical printing parameters and low nozzle shear stress. Human mesenchymal stem cells printed using CA as a bioink show significantly higher survival (95%) in comparison to when printed using native agarose (62%), a commonly used thermogelling hydrogel for 3D-bioprinting applications. This work paves the way toward the printing of complex tissue-like structures composed of a range of mechanically discrete microdomains that could potentially reproduce natural mechanical aspects of functional tissues.

Original languageEnglish
Article number1700255
Number of pages7
JournalAdvanced Healthcare Materials
Volume6
Issue number20
DOIs
Publication statusPublished - 25 Oct 2017
Externally publishedYes

Keywords

  • 3D printing
  • Bioprinting
  • Carboxylated agarose
  • Hydrogels
  • Mechanobiology

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