Measuring MIF in Biological Fluids

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Abstract

MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric F. Morand
Place of PublicationNew York
PublisherHumana Press
Chapter5
Pages47-56
Number of pages10
Volume2080
ISBN (Electronic)978-1-4939-9936-1_5
ISBN (Print)978-1-4939-9935-4
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MIF
  • Serum
  • Plasma
  • Urine
  • Cell culture
  • ELISA
  • Multiplex immunoassay
  • Quantibody®

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