Measuring MIF in Biological Fluids

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric F. Morand
Place of PublicationNew York
PublisherHumana Press
Chapter5
Pages47-56
Number of pages10
Volume2080
ISBN (Electronic)978-1-4939-9936-1_5
ISBN (Print)978-1-4939-9935-4
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MIF
  • Serum
  • Plasma
  • Urine
  • Cell culture
  • ELISA
  • Multiplex immunoassay
  • Quantibody®

Cite this

Vincent, F. B., & Lang, T. (2020). Measuring MIF in Biological Fluids. In J. Harris, & E. F. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (Vol. 2080, pp. 47-56). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_5
Vincent, Fabien Bernard ; Lang, Tali. / Measuring MIF in Biological Fluids. Macrophage Migration Inhibitory Factor: Methods and Protocols. editor / James Harris ; Eric F. Morand. Vol. 2080 New York : Humana Press, 2020. pp. 47-56 (Methods in Molecular Biology: Springer Protocols).
@inbook{1b974def00a548aab761502f722c7cf0,
title = "Measuring MIF in Biological Fluids",
abstract = "MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.",
keywords = "MIF, Serum, Plasma, Urine, Cell culture, ELISA, Multiplex immunoassay, Quantibody{\circledR}",
author = "Vincent, {Fabien Bernard} and Tali Lang",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9936-1_5",
language = "English",
isbn = "978-1-4939-9935-4",
volume = "2080",
series = "Methods in Molecular Biology: Springer Protocols",
publisher = "Humana Press",
pages = "47--56",
editor = "Harris, {James } and Morand, {Eric F.}",
booktitle = "Macrophage Migration Inhibitory Factor",
address = "United States of America",

}

Vincent, FB & Lang, T 2020, Measuring MIF in Biological Fluids. in J Harris & EF Morand (eds), Macrophage Migration Inhibitory Factor: Methods and Protocols. vol. 2080, Methods in Molecular Biology: Springer Protocols, vol. 2080, Humana Press, New York, pp. 47-56. https://doi.org/10.1007/978-1-4939-9936-1_5

Measuring MIF in Biological Fluids. / Vincent, Fabien Bernard; Lang, Tali.

Macrophage Migration Inhibitory Factor: Methods and Protocols. ed. / James Harris; Eric F. Morand. Vol. 2080 New York : Humana Press, 2020. p. 47-56 (Methods in Molecular Biology: Springer Protocols; Vol. 2080).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

TY - CHAP

T1 - Measuring MIF in Biological Fluids

AU - Vincent, Fabien Bernard

AU - Lang, Tali

PY - 2020/1/1

Y1 - 2020/1/1

N2 - MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

AB - MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

KW - MIF

KW - Serum

KW - Plasma

KW - Urine

KW - Cell culture

KW - ELISA

KW - Multiplex immunoassay

KW - Quantibody®

UR - http://www.scopus.com/inward/record.url?scp=85075308449&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9936-1_5

DO - 10.1007/978-1-4939-9936-1_5

M3 - Chapter (Book)

SN - 978-1-4939-9935-4

VL - 2080

T3 - Methods in Molecular Biology: Springer Protocols

SP - 47

EP - 56

BT - Macrophage Migration Inhibitory Factor

A2 - Harris, James

A2 - Morand, Eric F.

PB - Humana Press

CY - New York

ER -

Vincent FB, Lang T. Measuring MIF in Biological Fluids. In Harris J, Morand EF, editors, Macrophage Migration Inhibitory Factor: Methods and Protocols. Vol. 2080. New York: Humana Press. 2020. p. 47-56. (Methods in Molecular Biology: Springer Protocols). https://doi.org/10.1007/978-1-4939-9936-1_5