Abstract
MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.
| Original language | English |
|---|---|
| Title of host publication | Macrophage Migration Inhibitory Factor |
| Subtitle of host publication | Methods and Protocols |
| Editors | James Harris, Eric F. Morand |
| Place of Publication | New York |
| Publisher | Humana Press |
| Chapter | 5 |
| Pages | 47-56 |
| Number of pages | 10 |
| Volume | 2080 |
| ISBN (Electronic) | 978-1-4939-9936-1_5 |
| ISBN (Print) | 978-1-4939-9935-4 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
Publication series
| Name | Methods in Molecular Biology: Springer Protocols |
|---|---|
| Publisher | Springer |
| Volume | 2080 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Keywords
- MIF
- Serum
- Plasma
- Urine
- Cell culture
- ELISA
- Multiplex immunoassay
- Quantibody®
Cite this
}
Measuring MIF in Biological Fluids. / Vincent, Fabien Bernard; Lang, Tali.
Macrophage Migration Inhibitory Factor: Methods and Protocols. ed. / James Harris; Eric F. Morand. Vol. 2080 New York : Humana Press, 2020. p. 47-56 (Methods in Molecular Biology: Springer Protocols; Vol. 2080).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
TY - CHAP
T1 - Measuring MIF in Biological Fluids
AU - Vincent, Fabien Bernard
AU - Lang, Tali
PY - 2020/1/1
Y1 - 2020/1/1
N2 - MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.
AB - MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.
KW - MIF
KW - Serum
KW - Plasma
KW - Urine
KW - Cell culture
KW - ELISA
KW - Multiplex immunoassay
KW - Quantibody®
UR - http://www.scopus.com/inward/record.url?scp=85075308449&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9936-1_5
DO - 10.1007/978-1-4939-9936-1_5
M3 - Chapter (Book)
SN - 978-1-4939-9935-4
VL - 2080
T3 - Methods in Molecular Biology: Springer Protocols
SP - 47
EP - 56
BT - Macrophage Migration Inhibitory Factor
A2 - Harris, James
A2 - Morand, Eric F.
PB - Humana Press
CY - New York
ER -