Measuring autophagy in macrophages

James Harris, Orla Hanrahan, Sergio A de Haro

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Macroautophagy is a conserved intracellular homeostatic mechanism for the degradation of cytosolic constituents. Autophagy can promote cell survival by providing essential amino acids from the breakdown of macromolecules during periods of nutrient deprivation, and can remove damaged or excess organelles, such as mitochondria and peroxisomes. More recently, autophagy has been shown to play an important role in innate and adaptive immune responses to pathogenic bacteria in macrophages and dendritic cells. This unit presents protocols for the measurement of autophagy in macrophages.
Original languageEnglish
Pages (from-to)Unit 14.14 - Unit 14.14
Number of pages13
JournalCurrent Protocols in Immunology
VolumeChapter 14
DOIs
Publication statusPublished - 2009

Cite this

Harris, J., Hanrahan, O., & de Haro, S. A. (2009). Measuring autophagy in macrophages. Current Protocols in Immunology, Chapter 14, Unit 14.14 - Unit 14.14. https://doi.org/10.1002/0471142735.im1414s87
Harris, James ; Hanrahan, Orla ; de Haro, Sergio A. / Measuring autophagy in macrophages. In: Current Protocols in Immunology. 2009 ; Vol. Chapter 14. pp. Unit 14.14 - Unit 14.14.
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Harris, J, Hanrahan, O & de Haro, SA 2009, 'Measuring autophagy in macrophages' Current Protocols in Immunology, vol. Chapter 14, pp. Unit 14.14 - Unit 14.14. https://doi.org/10.1002/0471142735.im1414s87

Measuring autophagy in macrophages. / Harris, James; Hanrahan, Orla; de Haro, Sergio A.

In: Current Protocols in Immunology, Vol. Chapter 14, 2009, p. Unit 14.14 - Unit 14.14.

Research output: Contribution to journalArticleResearchpeer-review

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AB - Macroautophagy is a conserved intracellular homeostatic mechanism for the degradation of cytosolic constituents. Autophagy can promote cell survival by providing essential amino acids from the breakdown of macromolecules during periods of nutrient deprivation, and can remove damaged or excess organelles, such as mitochondria and peroxisomes. More recently, autophagy has been shown to play an important role in innate and adaptive immune responses to pathogenic bacteria in macrophages and dendritic cells. This unit presents protocols for the measurement of autophagy in macrophages.

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