Measurement of renal tissue oxygen tension: Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits

Chai Ling Leong, P M O'Connor, Gabriela Alejandra Eppel, Warwick Peter Anderson, Roger George Evans

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

BACKGROUND/AIMS: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. METHODS: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. RESULTS: Cortical oxygen tension varied with inspired O2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r(2) = 0.32; p <0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). CONCLUSION: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation.
Original languageEnglish
Pages (from-to)11 - 17
Number of pages7
JournalNephron Physiology
Volume108
Issue number2
Publication statusPublished - 2008

Cite this

@article{36e660ad7ca94b85b940849af92b1a89,
title = "Measurement of renal tissue oxygen tension: Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits",
abstract = "BACKGROUND/AIMS: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. METHODS: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. RESULTS: Cortical oxygen tension varied with inspired O2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r(2) = 0.32; p <0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). CONCLUSION: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation.",
author = "Leong, {Chai Ling} and O'Connor, {P M} and Eppel, {Gabriela Alejandra} and Anderson, {Warwick Peter} and Evans, {Roger George}",
year = "2008",
language = "English",
volume = "108",
pages = "11 -- 17",
journal = "Nephron",
issn = "1660-8151",
publisher = "S Karger AG",
number = "2",

}

Measurement of renal tissue oxygen tension: Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits. / Leong, Chai Ling; O'Connor, P M; Eppel, Gabriela Alejandra; Anderson, Warwick Peter; Evans, Roger George.

In: Nephron Physiology, Vol. 108, No. 2, 2008, p. 11 - 17.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Measurement of renal tissue oxygen tension: Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits

AU - Leong, Chai Ling

AU - O'Connor, P M

AU - Eppel, Gabriela Alejandra

AU - Anderson, Warwick Peter

AU - Evans, Roger George

PY - 2008

Y1 - 2008

N2 - BACKGROUND/AIMS: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. METHODS: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. RESULTS: Cortical oxygen tension varied with inspired O2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r(2) = 0.32; p <0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). CONCLUSION: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation.

AB - BACKGROUND/AIMS: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. METHODS: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. RESULTS: Cortical oxygen tension varied with inspired O2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r(2) = 0.32; p <0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). CONCLUSION: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18223307

M3 - Article

VL - 108

SP - 11

EP - 17

JO - Nephron

JF - Nephron

SN - 1660-8151

IS - 2

ER -