Measurement of penicillamine and N-acetylcysteine in human blood by high-performance liquid chromatography and electrochemical detection

Olaf H. Drummer, N. Christophidis, J. D. Horowitz, W. J. Louis

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A rapid and precise high-performance liquid chromatographic assay for both N-acetylcysteine and penicillamine in blood samples is described using selective reductive electrochemical detection and a high-efficiency C18 reversed-phase column. The use of an internal standard compensated for changes in detector responses during a run and for variable sample recovery. The detection limits for N-acetylcysteine and penicillamine were 25 and 10 ng/ml, respectively, using 500-μl blood samples. Reproducibility of measurement for both thiols was excellent. This method allows routine monitoring of blood levels and pharmacokinetic studies with N-acetylcysteine and penicillamine.

Original languageEnglish
Pages (from-to)251-257
Number of pages7
JournalJournal of Chromatography A
Issue number2
Publication statusPublished - 24 Jan 1986
Externally publishedYes

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