Measurement of oxidative stress: mitochondrial function using the seahorse system

Dilys T.H. Leung, Simon Chu

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

The Seahorse XFp Analyzer is a powerful tool for the assessment of various parameters of cellular respiration. Here we describe the process of the Seahorse Cell Phenotype Test using the Seahorse XFp Analyzer to characterize the metabolic phenotype of live cells. The Seahorse XFp Analyzer can also be coupled with other assays to measure cellular energetics. Given that mitochondrial dysfunction is implicated in preeclampsia, the Seahorse XFp Analyzer will serve as a useful tool for the understanding of pathological metabolism in this disorder.

Original languageEnglish
Title of host publicationPreeclampsia
Subtitle of host publicationMethods and Protocols
EditorsPadma Murthi, Cathy Vaillancourt
PublisherHumana Press
Chapter22
Pages285-293
Number of pages9
ISBN (Electronic)9781493974986
ISBN (Print)9781493974979
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1710
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Glycolysis
  • Metabolism
  • Mitochondrial function
  • Respiration
  • Seahorse mito-stress

Cite this

Leung, D. T. H., & Chu, S. (2018). Measurement of oxidative stress: mitochondrial function using the seahorse system. In P. Murthi, & C. Vaillancourt (Eds.), Preeclampsia: Methods and Protocols (pp. 285-293). (Methods in Molecular Biology; Vol. 1710). Humana Press. https://doi.org/10.1007/978-1-4939-7498-6_22
Leung, Dilys T.H. ; Chu, Simon. / Measurement of oxidative stress : mitochondrial function using the seahorse system. Preeclampsia: Methods and Protocols . editor / Padma Murthi ; Cathy Vaillancourt. Humana Press, 2018. pp. 285-293 (Methods in Molecular Biology).
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Leung, DTH & Chu, S 2018, Measurement of oxidative stress: mitochondrial function using the seahorse system. in P Murthi & C Vaillancourt (eds), Preeclampsia: Methods and Protocols . Methods in Molecular Biology, vol. 1710, Humana Press, pp. 285-293. https://doi.org/10.1007/978-1-4939-7498-6_22

Measurement of oxidative stress : mitochondrial function using the seahorse system. / Leung, Dilys T.H.; Chu, Simon.

Preeclampsia: Methods and Protocols . ed. / Padma Murthi; Cathy Vaillancourt. Humana Press, 2018. p. 285-293 (Methods in Molecular Biology; Vol. 1710).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Leung DTH, Chu S. Measurement of oxidative stress: mitochondrial function using the seahorse system. In Murthi P, Vaillancourt C, editors, Preeclampsia: Methods and Protocols . Humana Press. 2018. p. 285-293. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7498-6_22