Measurement of ligand-G protein-coupled receptor interactions

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

2 Citations (Scopus)
Original languageEnglish
Title of host publicationG Protein-Coupled Receptors: Essential Methods
EditorsDavid R Poyner, Mark Wheatley
Place of PublicationChichester UK
PublisherWiley-Blackwell
Pages1-29
Number of pages29
ISBN (Print)9780470749142
DOIs
Publication statusPublished - 25 Jan 2010

Publication series

NameProteins & Macromolecules
PublisherWiley-Blackwell

Keywords

  • Allosteric ligands
  • Hill-Langmuir binding isotherm
  • Ligand-G protein-coupled receptor (GPCR) interactions
  • Ligand-receptor interactions and law of mass action
  • Measurement of ligand-G protein-coupled receptor interactions
  • Protocol 1.2 saturation binding assays
  • Protocol 1.3 competition binding assays
  • Protocol 1.5 radioligand binding kinetics measurement
  • Radioligand binding assays
  • Radioligand dissociation kinetics in allosteric modulator presence

Cite this

Leach, K., Valant, C., Sexton, P. M., & Christopoulos, A. (2010). Measurement of ligand-G protein-coupled receptor interactions. In D. R. Poyner, & M. Wheatley (Eds.), G Protein-Coupled Receptors: Essential Methods (pp. 1-29). (Proteins & Macromolecules). Chichester UK: Wiley-Blackwell. https://doi.org/10.1002/9780470749210.ch1
Leach, Katie ; Valant, Celine ; Sexton, Patrick M ; Christopoulos, Arthur. / Measurement of ligand-G protein-coupled receptor interactions. G Protein-Coupled Receptors: Essential Methods. editor / David R Poyner ; Mark Wheatley. Chichester UK : Wiley-Blackwell, 2010. pp. 1-29 (Proteins & Macromolecules).
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Leach, K, Valant, C, Sexton, PM & Christopoulos, A 2010, Measurement of ligand-G protein-coupled receptor interactions. in DR Poyner & M Wheatley (eds), G Protein-Coupled Receptors: Essential Methods. Proteins & Macromolecules, Wiley-Blackwell, Chichester UK, pp. 1-29. https://doi.org/10.1002/9780470749210.ch1

Measurement of ligand-G protein-coupled receptor interactions. / Leach, Katie; Valant, Celine; Sexton, Patrick M; Christopoulos, Arthur.

G Protein-Coupled Receptors: Essential Methods. ed. / David R Poyner; Mark Wheatley. Chichester UK : Wiley-Blackwell, 2010. p. 1-29 (Proteins & Macromolecules).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

TY - CHAP

T1 - Measurement of ligand-G protein-coupled receptor interactions

AU - Leach, Katie

AU - Valant, Celine

AU - Sexton, Patrick M

AU - Christopoulos, Arthur

PY - 2010/1/25

Y1 - 2010/1/25

KW - Allosteric ligands

KW - Hill-Langmuir binding isotherm

KW - Ligand-G protein-coupled receptor (GPCR) interactions

KW - Ligand-receptor interactions and law of mass action

KW - Measurement of ligand-G protein-coupled receptor interactions

KW - Protocol 1.2 saturation binding assays

KW - Protocol 1.3 competition binding assays

KW - Protocol 1.5 radioligand binding kinetics measurement

KW - Radioligand binding assays

KW - Radioligand dissociation kinetics in allosteric modulator presence

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M3 - Chapter (Book)

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EP - 29

BT - G Protein-Coupled Receptors: Essential Methods

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A2 - Wheatley, Mark

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Leach K, Valant C, Sexton PM, Christopoulos A. Measurement of ligand-G protein-coupled receptor interactions. In Poyner DR, Wheatley M, editors, G Protein-Coupled Receptors: Essential Methods. Chichester UK: Wiley-Blackwell. 2010. p. 1-29. (Proteins & Macromolecules). https://doi.org/10.1002/9780470749210.ch1