mda-5, but not RIG-I, is a common target for paramyxovirus V proteins

Kay Childs, Nicola Stock, Craig Ross, Jelena Andrejeva, Louise Hilton, Michael Skinner, Richard Randall, Stephen Goodbourn

Research output: Contribution to journalArticleResearchpeer-review

234 Citations (Scopus)

Abstract

The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed.
Original languageEnglish
Pages (from-to)190 - 200
Number of pages11
JournalVirology
Volume359
Issue number1
Publication statusPublished - 2007
Externally publishedYes

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